Chemistry & Biochemistry Laboratory, Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
Chemistry & Biochemistry Laboratory, Department of Field and Vegetable Crops, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, Serbia.
Food Chem. 2021 Oct 1;358:129812. doi: 10.1016/j.foodchem.2021.129812. Epub 2021 Apr 19.
Prunus fruits are recognized to be rich sources of polyphenols with health promoting effect. In this work we evaluated the phenolic profile and bioactivity, namely antioxidant capacity, antiproliferative effect in HT29, and inhibition capacity of α-glucosidase (α-Gls), α-amylase (α-Amy) and human dipeptidyl peptidase III (hDPP III) activities, of traditional Prunus fruits grown in Serbia. Fifteen Prunus samples were investigated and compared: common European plum and three old plum subspecies ('vlaškača', damson plum and white damson), purple-leaf cherry plum, red and white cherry plum, sweet cherry, sweet cherry-wild type, sour cherry, steppe cherry, mahaleb cherry, blackthorn, peach, and apricot. Principal Component Analysis highlighted steppe cherry and blackthorn as Prunus species with the highest bioactive potential. In silico analysis pointed out rutinoside derivatives of cyanidin and quercetin as the most potent inhibitors of α-Gls, α-Amy and hDPP III enzymes. Quercetin 3-O-rutinoside showed the highest binding energy to α-Gls (-10.6 kcal/mol).
李属水果被认为是富含具有促进健康作用的多酚的来源。在这项工作中，我们评估了传统生长在塞尔维亚的李属水果的酚类成分和生物活性，包括抗氧化能力、对 HT29 的增殖抑制作用以及对α-葡萄糖苷酶（α-Gls）、α-淀粉酶（α-Amy）和人二肽基肽酶 III（hDPP III）活性的抑制能力。研究了 15 个李属水果样本，并进行了比较：常见的欧洲李和三个旧李亚种（'vlaškača'、西洋李和白西洋李）、紫叶樱桃李、红樱桃李和白樱桃李、甜樱桃、甜樱桃-野生型、酸樱桃、草原樱桃、野樱桃、桃和杏。主成分分析突出了草原樱桃和野樱桃是具有最高生物活性潜力的李属物种。计算机模拟分析指出，矢车菊素和槲皮素的芦丁衍生物是α-Gls、α-Amy 和 hDPP III 酶的最有效抑制剂。槲皮素 3-O-芦丁对 α-Gls 的结合能最高（-10.6 kcal/mol）。
