Sivas Cumhuriyet University, Department of Biochemistry, Sivas, Turkey.
Sivas Cumhuriyet University, Department of Chemistry, Sivas, Turkey.
Food Chem. 2021 Oct 1;358:129817. doi: 10.1016/j.foodchem.2021.129817. Epub 2021 Apr 19.
In this paper, a green and inexpensive air-assisted natural deep eutectic solvent-based solidified homogeneous liquid phase microextraction procedure was optimized for extraction of patulin in fruit juice and dried fruit samples using experimental design prior to its spectrophotometric determination. Four different natural deep eutectic solvent were prepared and applied to ensure efficient, and selective extraction of patulin. The significant variables including Zn(II) amount, cooling time, pH and amount of natural deep eutectic solvent were optimized by using central composite design. Under optimized conditions, working range was 10-750 μg L with 0.9996 of correlation coefficient. Detection limit and preconcentration factor were 3.5 μg L and 150, respectively. The repeatability and reproducibility precision were in the range of 3.2-4.6% and 4.3-5.6% respectively. Recoveries ranging from 94% to 104% proved the accuracy of the method. The optimized method was successfully applied to the extraction and identification of patulin in the selected samples.
本文采用实验设计优化了一种绿色、廉价的空气辅助天然深共晶溶剂固相亲和液相微萃取方法，用于果汁和干果样品中天马霉素的提取，然后进行分光光度法测定。制备了四种不同的天然深共晶溶剂，以确保对天冬霉素的有效、选择性提取。通过中心复合设计优化了 Zn(II)量、冷却时间、pH 值和天然深共晶溶剂用量等显著变量。在优化条件下，工作范围为 10-750μg/L，相关系数为 0.9996。检测限和预浓缩因子分别为 3.5μg/L 和 150。重复性和重现性精度分别在 3.2-4.6%和 4.3-5.6%范围内。回收率在 94%-104%之间，证明了该方法的准确性。该优化方法成功应用于所选样品中天马霉素的提取和鉴定。
