Institute of Bioengineering and Medical Engineering, Guangdong Academy of Sciences, Guangzhou, 510316, People's Republic of China.
Guangdong Engineering Research Center for Sugar Technology, Guangzhou, 510316, People's Republic of China.
Anal Bioanal Chem. 2022 May;414(11):3497-3505. doi: 10.1007/s00216-022-03970-3. Epub 2022 Feb 15.
A novel method was developed to determine six triazine herbicides from brown sugar samples using matrix solid-phase dispersion (MSPD) based on silica gel impregnated with deep eutectic solvent (DES) followed by high-performance liquid chromatography with photodiode array detector (HPLC/PDA). Several factors involved in the MSPD procedure such as DES type, DES content in impregnated silica gel, adsorbent-to-sample mass ratio, type and volume of washing solvent, type and volume of eluent, and grinding time were screened using single-factor experiments and then optimized using Box-Behnken design to accomplish the highest recoveries. The above method demonstrated a good linear range (20-1000 μg kg) with a determination coefficient exceeding 0.9962, low limits of determination (1.59-3.77 μg kg), acceptable limits of quantifications, and acceptable spiking recoveries (95.0-101.7%) for six triazines under optimized conditions. The proposed MSPD-HPLC/PDA method is a convenient, effective, and sensitive method for rapidly isolating and quantifying six triazines from brown sugar.
建立了一种基于硅胶浸渍深共晶溶剂（DES）的新型基质固相分散（MSPD）方法，结合高效液相色谱-光电二极管阵列检测（HPLC/PDA），用于从红糖样品中检测六种三嗪类除草剂。通过单因素实验筛选了 MSPD 过程中的几个因素，如 DES 类型、浸渍硅胶中的 DES 含量、吸附剂与样品的质量比、洗涤溶剂的类型和体积、洗脱剂的类型和体积以及研磨时间，然后使用 Box-Behnken 设计进行优化，以实现最高的回收率。在优化条件下，该方法对六种三嗪类除草剂表现出良好的线性范围（20-1000μgkg），相关系数均大于 0.9962，检测限低（1.59-3.77μgkg），定量限可接受，加标回收率（95.0-101.7%）也令人满意。该 MSPD-HPLC/PDA 方法是一种简便、有效、灵敏的方法，可快速从红糖中分离和定量六种三嗪类除草剂。
