《广盐轮虫 Brachionus paranguensis 的基因组：在分子生态毒理学中的潜在应用》。
The genome of the euryhaline rotifer Brachionus paranguensis: Potential use in molecular ecotoxicology.
机构信息
Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki 852-8521, Japan; Organization for Marine Science and Technology, Nagasaki University, Nagasaki 852-8521, Japan.
出版信息
Comp Biochem Physiol Part D Genomics Proteomics. 2021 Sep;39:100836. doi: 10.1016/j.cbd.2021.100836. Epub 2021 Apr 18.
Brachionus spp. rotifers have been proposed as model organisms for ecotoxicological studies. We analyzed the whole-genome sequence of B. paranguensis through NextDenovo, resulting in a total length of 106.2 Mb and 71 contigs. The N50 and the GC content were 4.13 Mb and 28%, respectively. A total of 18,501 genes were predicted within the genome of B. paranguensis. Prominent detoxification-related gene families of phase I and II detoxifications have been investigated. In parallel with other Brachionus rotifers, high gene expansion was observed in CYP clan 3 and GST sigma class in B. paranguensis. Moreover, species-specific expansion of sulfotransferase (SULTs) and gain of UDP-glucuronosyltransferases (UGTs) through horizontal gene transfer has been specifically found within B. plicatilis complex. This whole-genome analysis of B. paranguensis provides a basis for molecular ecotoxicological studies and provides useful information for comparative studies of the evolution of detoxification mechanisms in Brachionus spp.
臂尾轮虫已被提议作为生态毒理学研究的模式生物。我们通过 NextDenovo 对 B. paranguensis 的全基因组序列进行了分析，结果得到了总长为 106.2Mb 的 71 个连续序列。N50 和 GC 含量分别为 4.13Mb 和 28%。在 B. paranguensis 的基因组中预测到了 18501 个基因。研究了明显与解毒相关的一期和二期解毒相关基因家族。与其他臂尾轮虫一样，CYP 族 3 和 GST sigma 类在 B. paranguensis 中观察到了高基因扩张。此外，在 B. plicatilis 复合体中还发现了通过水平基因转移获得的磺基转移酶（SULTs）和 UDP-葡糖醛酸基转移酶（UGTs）的物种特异性扩张。B. paranguensis 的全基因组分析为分子生态毒理学研究提供了基础，并为臂尾轮虫属解毒机制进化的比较研究提供了有用信息。
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