Biomedical Research Center, Korea University Ansan Hospital, Gyeonggi-do, Republic of Korea; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea; Zebrafish Translational Medical Research Center, Korea University, Ansan, Gyeonggi-do, Republic of Korea; Incheon Technopark Bioindustry Center, Incheon, Republic of Korea.
Zebrafish Translational Medical Research Center, Korea University, Ansan, Gyeonggi-do, Republic of Korea; Department of Ophthalmology, Korea University College of Medicine, Seoul, Republic of Korea; Department of Ophthalmology, Korea University Ansan Hospital, Gyeonggi-do, Republic of Korea.
Biochem Biophys Res Commun. 2021 Jun 25;559:155-160. doi: 10.1016/j.bbrc.2021.04.102. Epub 2021 Apr 30.
To investigate the efficacy of a novel experimental model for exploring visual function using a contrast-optomotor response (C-OMR) assay made by applying the contrast sensitivity test to the OMR assay in zebrafish.
Zebrafish larvae were treated with 0 (control), 5, 10, or 15 μM gentamicin and digoxin for 24 h at four days post-fertilization (dpf). Zebrafish larvae were assessed using the C-OMR assay with graded contrast gray-white stripes at 5 dpf, and the results were expressed as the percentage of larvae that finished swimming for 30 s (n = 20 per each group). The same C-OMR assay was repeated four times using different larvae.
The percentage of larvae that finished swimming within 30 s was significantly reduced in larvae treated with 5, 10, and 15 μM gentamicin and 10 and 15 μM digoxin as compared to the Control groups. The C-OMR assay could distinguish that the decrease in visual function was different depending on the concentration of gentamicin and digoxin (5, 10, and 15 μM), whereas the OMR test with one contrast gray-white stripe could not.
The method of analyzing zebrafish OMR using graded contrast gray-white stripes is more sensitive than the OMR assay alone and may be more useful for assessing the drug toxicity and eye-related diseases to improve the understanding of drug-induced ocular side effects in the clinic.
为了研究一种新型的实验模型，该模型通过将对比敏感度测试应用于斑马鱼的光反应（OMR）测试，来探索视觉功能。
在受精后 4 天（dpf），用 0（对照）、5、10 或 15μM 庆大霉素和地高辛处理斑马鱼幼虫 24 小时。在 5 dpf 时，使用灰度对比光反应（C-OMR）测试评估斑马鱼幼虫，结果表示为 30 秒内完成游泳的幼虫百分比（每组 20 个）。使用不同的幼虫重复进行了四次相同的 C-OMR 测试。
与对照组相比，用 5、10 和 15μM 庆大霉素以及 10 和 15μM 地高辛处理的幼虫中，30 秒内完成游泳的幼虫百分比显著降低。C-OMR 测试可以区分视觉功能的下降程度因庆大霉素和地高辛的浓度而异（5、10 和 15μM），而单一灰度对比的 OMR 测试则不能。
使用灰度对比光反应分析斑马鱼 OMR 的方法比单独使用 OMR 测试更敏感，可能更有助于评估药物毒性和眼部相关疾病，从而加深对临床中药物引起的眼部副作用的认识。
