Kanani Fatima Husain, Hussain Tahir, Talat Tayyaba, Ghouri Nida
The Indus Hospital, Karachi, Pakistan.
J Pak Med Assoc. 2021 Feb;71(2(B)):708-712. doi: 10.47391/JPMA.1301.
To analyse frequencies and results of anti-nuclear antibodies, anti-double stranded deoxyribonucleic acid and anti-extractable nuclear antigens tests ordered in a tertiary-care hospital.
The retrospective study was conducted at a tertiary care hospital in Karachi, and comprised all tests ordered for anti-nuclear antibodies, anti-double stranded deoxyribonucleic acid and anti-extractable nuclear antigens from March 2017 to January 2018. Data was retrieved from the institutional electronic database. The frequencies and results of the tests were determined. Anti-nuclear antibodies test was determined by indirect immunofluorescence, while the other two tests were determined by enzyme-linked immunosorbent assay. Patterns emerging from anti-nuclear antibodies tests were also analysed.
Of the 1053 cases studied, 1000(95%) were tested for for anti-nuclear antibodies. The test was positive in 260(26%) patients, and was repeated in 8(3%) of the positive and 9(1.2%) of the negative patients. Anti-double stranded deoxyribonucleic acid test was ordered in 300(40.5%) and anti-extractable nuclear antigens test in 125(17%) patients who had tested negative for anti-nuclear antibodies. Among those who tested positive for anti-nuclear antibodies, the commonly observed patterns were homogenous 109(41.9%) and speckled 103(39.6%). Rod and ring pattern was seen in 10(3.8%) patients, and none of them were on anti-viral treatment.
There was injudicious and unjustified ordering of auto-antibodies testing, indicating the need for greater physician education and cost-effective protocols.
分析在一家三级医疗医院进行的抗核抗体、抗双链脱氧核糖核酸及抗可提取核抗原检测的频率及结果。
该回顾性研究在卡拉奇的一家三级医疗医院开展，纳入了2017年3月至2018年1月期间所有进行的抗核抗体、抗双链脱氧核糖核酸及抗可提取核抗原检测。数据从机构电子数据库中获取。确定检测的频率及结果。抗核抗体检测采用间接免疫荧光法，另外两项检测采用酶联免疫吸附测定法。还分析了抗核抗体检测中出现的模式。
在研究的1053例病例中，1000例（95%）进行了抗核抗体检测。260例（26%）患者检测结果为阳性，其中8例（3%）阳性患者及9例（1.2%）阴性患者进行了复测。抗核抗体检测呈阴性的患者中，300例（40.5%）进行了抗双链脱氧核糖核酸检测，125例（17%）进行了抗可提取核抗原检测。在抗核抗体检测呈阳性的患者中，常见模式为均质型109例（41.9%）和斑点型103例（39.6%）。10例（3.8%）患者出现了棒状和环状模式，且均未接受抗病毒治疗。
自身抗体检测存在不合理和无正当理由的开单情况，表明需要加强医生教育并制定具有成本效益的方案。
