Ramanujam Ashwin, Neyhouse Bertrand, Keogh Rebecca A, Muthuvel Madhivanan, Carroll Ronan K, Botte Gerardine G
Chemical and Electrochemical Technology and Innovation Laboratory, Department of Chemical Engineering, Texas Tech University, Lubbock, TX 79409, USA.
Center for Electrochemical Engineering Research, Department of Chemical and Biomolecular Engineering, Ohio University, Athens, OH 45701, USA.
Chem Eng J. 2021 May 1;411:128453. doi: 10.1016/j.cej.2021.128453.
A standalone electrochemical method for detecting the bacterium in water was developed using a nickel electrode and no biorecognition element. Electric current responses from different concentrations were recorded based on their interaction with a locally formed electrocatalyst. A rotating disk electrode was used to minimize the mass transport limitations at the interface. Results from experiments with the rotating disk electrode also paved the way for hypothesizing the detection mechanism. The operating conditions were established for sensing the electric current responses in the presence of . The least-squares linear regression model was fit to the data obtained from currents of some known concentrations. This probe had a detection limit in the order of 10 CFU/ml. The response time to detect the presence/absence of was less than half a second, while the total assay time, including quantification of its concentration, was 10 min. The electric current response from a solution mixed with and showed current similar to only solution indicating the specificity of the sensor to respond to signals from . This electrochemical microbial sensor's uniqueness lies in its ability to rapidly detect by forming the catalyst locally on demand without the attachment of biorecognition elements.
开发了一种独立的电化学方法，用于检测水中的细菌，该方法使用镍电极且不使用生物识别元件。基于不同浓度的细菌与局部形成的电催化剂的相互作用，记录了电流响应。使用旋转圆盘电极来最小化界面处的传质限制。旋转圆盘电极的实验结果也为推测检测机制铺平了道路。确定了在存在[具体物质未给出]的情况下传感电流响应的操作条件。将最小二乘线性回归模型拟合到从一些已知浓度的电流获得的数据。该探针的检测限约为10 CFU/ml。检测[具体细菌未给出]存在与否的响应时间不到半秒，而包括其浓度定量在内的总检测时间为10分钟。与[具体细菌未给出]和[具体物质未给出]混合的溶液的电流响应显示出与仅含[具体细菌未给出]溶液相似的电流，表明该传感器对来自[具体细菌未给出]的信号有特异性响应。这种电化学微生物传感器的独特之处在于它能够通过按需在局部形成催化剂而无需附着生物识别元件来快速检测[具体细菌未给出]。
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