Sharma Arti, Mishra Akanksha, Chhabra Meenu
Environmental Biotechnology Laboratory, Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur (IITJ), Jodhpur, 342030, Rajasthan, India.
Heliyon. 2024 Feb 21;10(5):e26724. doi: 10.1016/j.heliyon.2024.e26724. eCollection 2024 Mar 15.
The present study describes the development of a potentiometric sensor for microbial monitoring in water based on catalase activity. The sensor comprises a MnO-modified electrode that responds linearly to hydrogen peroxide (HO) from 0.16 M to 3.26 M. The electrode potential drops when the HO solution is spiked with catalase or catalase-producing microorganisms that decompose HO. The sensor is responsive to different bacteria and their catalase activities. The electrochemical sensor exhibits a lower limit of detection (LOD) for at 11 CFU/ml, at 12 CFU/ml, and at 23 CFU/ml. The sensor shows high sensitivity at 3.49, 3.02, and 4.24 mV/cmdec for , , and respectively. The abiotic sensing electrode can be used multiple times without changing the response potential (up to 100 readings) with a shelf-life of over six months. The response time is a few seconds, with a total test time of 5 min. Additionally, the sensor effectively tested actual samples (drinking and grey water), which makes it a quick and reliable sensing tool. Therefore, the study offers a promising water monitoring tool with high sensitivity, stability, good detection limit, and minimum interference from other water contaminants.
本研究描述了一种基于过氧化氢酶活性用于水中微生物监测的电位传感器的开发。该传感器包括一个MnO修饰电极，其对过氧化氢（H₂O₂）在0.16 M至3.26 M范围内呈线性响应。当H₂O₂溶液中加入分解H₂O₂的过氧化氢酶或产过氧化氢酶微生物时，电极电位会下降。该传感器对不同细菌及其过氧化氢酶活性有响应。该电化学传感器对大肠杆菌的检测下限（LOD）为11 CFU/ml，对金黄色葡萄球菌为12 CFU/ml，对铜绿假单胞菌为23 CFU/ml。该传感器对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的灵敏度分别为3.49、3.02和4.24 mV/cmdec。这种非生物传感电极可以多次使用而不改变响应电位（最多可读取100次），保质期超过六个月。响应时间为几秒，总测试时间为5分钟。此外，该传感器有效地测试了实际样品（饮用水和中水），这使其成为一种快速可靠的传感工具。因此，该研究提供了一种具有高灵敏度、稳定性、良好检测限且受其他水污染物干扰最小的有前景的水监测工具。
