Mutlaq Sawsan, Albiss Borhan, Al-Nabulsi Anas A, Jaradat Ziad W, Olaimat Amin N, Khalifeh Mohammad S, Osaili Tareq, Ayyash Mutamed M, Holley Richard A
Department of Nutrition and Food Technology, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.
Nanomaterials Laboratory, Department of Applied Physics, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.
Polymers (Basel). 2021 Sep 26;13(19):3288. doi: 10.3390/polym13193288.
A conductometric immunosensor was developed for the detection of one of the most common foodborne pathogens, O157:H7 ( O157:H7), by conductometric sensing. The sensor was built based on a polyaniline/zinc oxide (PANI/ZnO) nanocomposite film spin-coated on a gold electrode. Then, it was modified with a monoclonal anti- O157:H7 antibody as a biorecognition element. The fabricated nanostructured sensor was able to quantify the pathogens under optimal detection conditions, within 30 min, and showed a good detection range from 10 to 10 CFU/mL for O157:H7 and a minimum detection limit of 4.8 CFU/mL in 0.1% peptone water. The sensor efficiency for detecting bacteria in food matrices was tested in ultra-heat-treated (UHT) skim milk. O157:H7 was detected at concentrations of 10 to 10 CFU/mL with a minimum detection limit of 13.9 CFU/mL. The novel sensor was simple, fast, highly sensitive with excellent specificity, and it had the potential for rapid sample processing. Moreover, this unique technique for bacterial detection could be applicable for food safety and quality control in the food sector as it offers highly reliable results and is able to quantify the target bacterium.
通过电导传感技术开发了一种用于检测最常见的食源性病原体之一O157:H7的电导免疫传感器。该传感器基于旋涂在金电极上的聚苯胺/氧化锌（PANI/ZnO）纳米复合膜构建。然后，用单克隆抗O157:H7抗体作为生物识别元件对其进行修饰。所制备的纳米结构传感器能够在最佳检测条件下30分钟内对病原体进行定量，对O157:H7的检测范围为10至10 CFU/mL，在0.1%蛋白胨水中的最低检测限为4.8 CFU/mL。在超高温处理（UHT）脱脂牛奶中测试了该传感器检测食品基质中细菌的效率。检测到的O157:H7浓度为10至10 CFU/mL，最低检测限为13.9 CFU/mL。这种新型传感器简单、快速、高度灵敏且具有出色的特异性，具有快速样品处理的潜力。此外，这种独特的细菌检测技术可应用于食品行业的食品安全和质量控制，因为它能提供高度可靠的结果并能够对目标细菌进行定量。
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