School of Stomatology, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
Department of Oral Anatomy and Physiology, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
Eur J Oral Sci. 2021 Aug;129(4):e12788. doi: 10.1111/eos.12788. Epub 2021 May 4.
Occlusion has been proposed to play a role for body posture and balance, both of which are mediated mainly by the cerebellum. The dorsomedial part of the principal sensory trigeminal nucleus (Vpdm) has direct projection to the cerebellum. The experimental unilateral anterior crossbite (UAC) has an impact on the motor nuclei in the brain stem via trigeminal mesencephalic nucleus (Vme). The current aim was to explore whether UAC has an impact on Vpdm-cerebellum circuit. The inferior alveolar nerve was injected into cholera toxin B subunit (CTb), the cerebellum was injected into fluoro-gold (FG), and the Vpdm was injected into biotinylated dextran amine (BDA) to identify the activation of Vpdm-cerebellum circuit by UAC. Data indicated that there were more neuronal nuclei (NeuN)/CTb/FG triple-labelled neurons and NeuN/CTb/vesicular glutamate transporter 1(VGLUT1) triple-labelled neurons in the Vpdm, and more NeuN/BDA/ VGLUT1 triple-labelled neurons in the cerebellum of rats with UAC than in control rats. The VGLUT1 expression in the Vpdm and cerebellum in the UAC group was higher than that in control rats. These findings indicate an excitatory impact of UAC on the Vpdm-cerebellum pathway and support the role of occlusion for body posture and balance.
阻塞被认为在姿势和平衡中发挥作用，而这两者主要由小脑介导。主要感觉三叉神经核背内侧部分（Vpdm）有直接投射到小脑。实验性单侧前交叉咬合（UAC）通过三叉神经中脑核（Vme）对脑干中的运动核产生影响。目前的目的是探讨 UAC 是否对 Vpdm-小脑回路有影响。在下牙槽神经中注射霍乱毒素 B 亚单位（CTb），在小脑内注射荧光金（FG），并在 Vpdm 内注射生物素化葡聚糖胺（BDA），以确定 UAC 对 Vpdm-小脑回路的激活作用。数据表明，UAC 大鼠的 Vpdm 中有更多的神经元核（NeuN）/CTb/FG 三标神经元和 NeuN/CTb/囊泡谷氨酸转运体 1（VGLUT1）三标神经元，而小脑中有更多的 NeuN/BDA/VGLUT1 三标神经元。UAC 组 Vpdm 和小脑中的 VGLUT1 表达高于对照组。这些发现表明 UAC 对 Vpdm-小脑通路有兴奋作用，并支持咬合在姿势和平衡中的作用。
