Physiology Program, Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hatyai Campus, Hatyai, Songkhla, 90110, Thailand; Biosignal Research Center for Health, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand.
Biology Program, Division of Biological Science, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand; Biosignal Research Center for Health, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla, 90112, Thailand.
Neurosci Lett. 2021 Jun 21;756:135939. doi: 10.1016/j.neulet.2021.135939. Epub 2021 May 1.
The basolateral amygdala (BLA) plays a crucial role in conditioned place preference (CPP) for addictive drugs. However, neural signaling associated with methamphetamine (METH) craving and seeking remained to be investigated. This study characterized local field potential (LFP) oscillatory patterns in the BLA and conditioned place preference induced by METH-related context. Male Swiss albino ICR mice were deeply anesthetized for LFP intracranial electrode implantation in the BLA. Control and METH groups received sessions to learn to associate saline-paired and METH-paired compartments of the CPP apparatus with saline and METH injections, respectively, for 10 days. LFP signals and exploring behavior were recorded simultaneously during pre- and post-conditioning phases. Time spent in METH-paired compartment was normalized and expressed as CPP scores. Fast Fourier Transform (FFT) algorithm was used to analyze LFP powers of 8 discrete frequency ranges (delta, theta, alpha, beta, gamma I-IV). During post-conditioning phase of METH CPP with METH cues, statistical analysis revealed that METH group significantly increased time spent in METH-paired compartment. Significant suppressions of theta and alpha powers were observed. Phase-amplitude cross frequency coupling analyses confirmed significant increases in maximal modulation index (MI), frequency for phase of slow wave and MI of theta-gamma II coupling. Taken together, LFP oscillation in the BLA was sensitive in association with METH CPP. These research findings might suggest the underlying mechanisms of drug reward learning and adaptive changes in the BLA in acquisition of METH CPP and dependence.
外侧杏仁核（BLA）在药物成瘾的条件性位置偏好（CPP）中起着至关重要的作用。然而，与甲基苯丙胺（METH）渴望和寻求相关的神经信号仍有待研究。本研究描述了 BLA 中的局部场电位（LFP）振荡模式以及与 METH 相关的环境引起的条件性位置偏好。雄性瑞士白化 ICR 小鼠在深度麻醉下进行 LFP 颅内电极植入 BLA。对照组和 METH 组接受了 10 天的学习，以将盐水配对和 METH 配对的 CPP 仪器室与盐水和 METH 注射分别关联起来。在预处理和后处理阶段同时记录 LFP 信号和探索行为。在 METH 配对室中花费的时间被归一化并表示为 CPP 分数。快速傅里叶变换（FFT）算法用于分析 8 个离散频率范围（δ、θ、α、β、γ I-IV）的 LFP 功率。在 METH CPP 与 METH 线索的后处理阶段，统计分析显示 METH 组在 METH 配对室中花费的时间显著增加。观察到θ和α功率的显著抑制。相位-幅度交叉频率耦合分析证实了最大调制指数（MI）、慢波相位频率和θ-γ II 耦合 MI 的显著增加。总之，BLA 中的 LFP 振荡与 METH CPP 相关，敏感性较高。这些研究结果可能表明了药物奖励学习和 BLA 适应变化的潜在机制，在获得 METH CPP 和依赖方面。
