Department of Forensic Medicine, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi, PR China.
Physiol Behav. 2013 Jun 13;118:195-200. doi: 10.1016/j.physbeh.2013.05.034. Epub 2013 May 25.
Levo-tetrahydropalmatine (l-THP), as an alkaloid purified from the traditional Chinese herbal medicine Corydalis and Stephania, has been widely used to produce many traditional Chinese herbal preparations. The effect of l-THP on methamphetamine-induced reward learning still remains unclear although it has been proved to be effective on treating allodynia and drug addiction. This experiment has been designed to examine the effect of l-THP on the acquisition, expression, extinction, and reinstatement of methamphetamine-induced conditioned place preference (CPP) in mice. The results show that methamphetamine (METH) could induce CPP in mice at doses of 0.5mg/kg, 1.0mg/kg and 2.0mg/kg respectively, but l-THP alone could not do so. Meanwhile, l-THP could not induce conditioned place aversion at doses of 1.25mg/kg to 20.0mg/kg in mice, but it could attenuate the acquisition and expression of METH-induced CPP and facilitate the extinction of METH-induced CPP in mice. Besides, l-THP could inhibit the reinstatement of METH-induced CPP at the dose of 10.0mg/kg whether it was given in the extinction training phase or 30min before the reinstatement. These results suggest that l-THP can globally suppress the rewarding properties of METH on all phases of the CPP task and it may have potential effects on the treatment of METH abuse.
左旋延胡索乙素（l-THP）作为从传统中药延胡索和千金藤中提取的生物碱，已被广泛用于生产许多中药制剂。尽管左旋延胡索乙素已被证明对治疗痛觉过敏和药物成瘾有效，但它对甲基苯丙胺引起的奖励学习的影响仍不清楚。本实验旨在研究左旋延胡索乙素对小鼠甲基苯丙胺诱导的条件位置偏爱（CPP）的获得、表达、消退和复燃的影响。结果表明，甲基苯丙胺（METH）可在 0.5mg/kg、1.0mg/kg 和 2.0mg/kg 剂量下诱导小鼠 CPP，但左旋延胡索乙素单独使用则不能。同时，左旋延胡索乙素在 1.25mg/kg 至 20.0mg/kg 剂量下不能诱导小鼠产生条件性位置厌恶，但能减弱 METH 诱导的 CPP 的获得和表达，并促进 METH 诱导的 CPP 的消退。此外，左旋延胡索乙素在 10.0mg/kg 剂量下能抑制 METH 诱导的 CPP 的复燃，无论它是在消退训练阶段还是在复燃前 30 分钟给予。这些结果表明，左旋延胡索乙素可以全面抑制 METH 在 CPP 任务的所有阶段的奖赏特性，它可能对治疗 METH 滥用具有潜在的效果。
