School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China.
School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China.
J Biotechnol. 2021 Jun 10;333:39-48. doi: 10.1016/j.jbiotec.2021.04.011. Epub 2021 May 1.
The quality of compost is drastically reduced due to the loss of carbon, which negatively impacts the environment. Carbon emission reduction and carbon dioxide (CO) fixation have attracted much attention in composting research. In this study, the relationship between CO emission, humic substances (HS) formation and cbbL-containing autotrophic microbes (CCAM) was analyzed by adding biochar during cow manure composting. The results showed that biochar can facilitate the degradation of organic matter (OM) and formation of HS, as well as reinforce the diversity and abundance of CCAM community, thereby promoting CO fixation and reducing carbon loss during composting. High-throughput sequencing analysis revealed significant increase in Actinobacteriota and Proteobacteria abundance by 30.97 % and 10.48 %, respectively, thus increasing carbon fixation by 32.07 %. Additionally, Alpha diversity index increased significantly during thermophilic phase, while Shannon index increased by 143.12 % and Sobs index increased by 51.62 %. Redundancy analysis (RDA) indicated that CO was positively correlated with C/N, temperature, HS and dissolved organic matter (DOM), while the abundance of Paeniclostridium, Corynebacterium, Bifidobacterium, Clostridium, Turicibacter and Romboutsia were positively correlated with temperature, CO, C/N and E/E (p < 0.01).
由于碳的损失，堆肥的质量大大降低，这对环境产生负面影响。在堆肥研究中，减少碳排放和固定二氧化碳（CO）受到了广泛关注。在这项研究中，通过在牛粪堆肥过程中添加生物炭，分析了 CO 排放、腐殖质（HS）形成和含有 cbbL 的自养微生物（CCAM）之间的关系。结果表明，生物炭可以促进有机物（OM）的降解和 HS 的形成，并增强 CCAM 群落的多样性和丰度，从而促进 CO 的固定和堆肥过程中碳的损失减少。高通量测序分析显示，放线菌门和变形菌门的丰度分别增加了 30.97%和 10.48%，从而增加了 32.07%的碳固定。此外，在高温阶段，Alpha 多样性指数显著增加，Shannon 指数增加了 143.12%，Sobs 指数增加了 51.62%。冗余分析（RDA）表明，CO 与 C/N、温度、HS 和溶解有机物质（DOM）呈正相关，而 Paeniclostridium、Corynebacterium、Bifidobacterium、Clostridium、Turicibacter 和 Romboutsia 的丰度与温度、CO、C/N 和 E/E 呈正相关（p < 0.01）。
