John W. Deming Department of Medicine, Section of Pulmonary Diseases, Tulane Eosinophilic Disorder Center (TEDC), Tulane University School of Medicine, New Orleans, LA, USA.
Allergy and Immunology Division, Cincinnati Children's Hospital Medical Center (CCHMC), Cincinnati, OH 45229, USA.
Clin Immunol. 2021 Jun;227:108752. doi: 10.1016/j.clim.2021.108752. Epub 2021 May 1.
Eosinophilic esophagitis (EoE) is often misdiagnosed as GERD; therefore, the goal of the current study is to establish a non-invasive diagnostic and monitoring biomarker that differentiated GERD from EoE. Reports indicates that IL-15 responsive iNKT cells and tissue specific IgE have a critical in EoE pathogenesis, not in GERD. Therefore, we tested the hypothesis that the panel of IL-15-responsive T cell and IgE receptors may be novel non-invasive biomarkers for EoE. Accordingly, the receptors of IL-15 responsive T cells (Vα24, Jα18, γδT, αβT) and IgE (FcεRI & FcεRII) were examined. The data indicates that blood mRNA levels of Vα24, Jα18, γδ T, αβ T and FcεRI are significantly reduced in EoE compared to the GERD patients and normal individuals. The ROC curve analysis indicated FcεRII, Jα18 and δ TCR are the positive predictors that discriminate EoE from GERD. Thus, these molecules will be a novel non-invasive diagnostic biomarker for EoE.
嗜酸性食管炎（EoE）常被误诊为 GERD；因此，本研究的目的是建立一种非侵入性的诊断和监测生物标志物，以区分 GERD 和 EoE。有报道称，IL-15 反应性 iNKT 细胞和组织特异性 IgE 在 EoE 的发病机制中起关键作用，而在 GERD 中则不起作用。因此，我们检验了这样一个假设，即 IL-15 反应性 T 细胞和 IgE 受体的组合可能是 EoE 的新型非侵入性生物标志物。因此，我们检查了 IL-15 反应性 T 细胞（Vα24、Jα18、γδT、αβT）和 IgE（FcεRI 和 FcεRII）的受体。数据表明，与 GERD 患者和正常人相比，EoE 患者血液中 Vα24、Jα18、γδ T、αβ T 和 FcεRI 的 mRNA 水平显著降低。ROC 曲线分析表明，FcεRII、Jα18 和 δ TCR 是区分 EoE 和 GERD 的阳性预测因子。因此，这些分子将是 EoE 的一种新型非侵入性诊断生物标志物。
