A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia.
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia.
Food Chem. 2021 Oct 15;359:129927. doi: 10.1016/j.foodchem.2021.129927. Epub 2021 Apr 22.
In this study, we developed a lateral flow immunoassay (LFIA) for the detection of pork additives in meat products. LFIA of porcine immunoglobulins (IgG) as a molecular biomarker was carried out in a sandwich format for species identification. Gold nanoparticles as a nano-dispersed label were conjugated to secondary antibodies specific to anti-porcine IgG. The test system was characterized by high specificity, which was confirmed by the absence of cross-reactivity with any other species tested. A short technique of sample preparation was proposed aimed at the effective extraction of IgG from meat samples. The developed LFIA enabled the detection of a pork ingredient at a level as low as 0.063% (w/w) in raw meat within 35 min including sample preparation. A large panel of real meat samples was analyzed by the LFIA. The results showed that porcine IgG can be reliably recognized both in raw meat products and processed meat foodstuffs.
在这项研究中，我们开发了一种用于检测肉类产品中猪肉添加剂的侧向流动免疫分析（LFIA）。采用夹心模式对猪免疫球蛋白（IgG）作为分子生物标志物进行 LFIA，用于物种鉴定。金纳米颗粒作为纳米分散标签与针对抗猪 IgG 的二级抗体偶联。该测试系统具有高度特异性，这一点通过与任何其他测试物种均无交叉反应得到证实。提出了一种简短的样品制备技术，旨在有效提取肉样中的 IgG。开发的 LFIA 能够在包括样品制备在内的 35 分钟内，在生肉中低至 0.063%（w/w）的猪肉成分水平下进行检测。利用 LFIA 分析了大量实际肉样。结果表明，猪 IgG 可以在生肉产品和加工肉食品中可靠识别。
