A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia.
V.M. Gorbatov Federal Research Center for Food Systems of the Russian Academy of Sciences, Talalikhina Street 26, Mocow 109316, Russia.
Food Chem. 2021 May 15;344:128598. doi: 10.1016/j.foodchem.2020.128598. Epub 2020 Nov 11.
This study presents the development of an immunochromatographic test system aimed at the detection of chicken additives in meat products. It is based on sandwich-format lateral flow immunoassay (LFIA) of immunoglobulins as a biomarker for species identification. The LFIA based on gold nanoparticles as a label for anti-species antibodies was used to determine chicken immunoglobulins and, accordingly, chicken meat in food products. Absence of cross-reactivity with mammalian species tested in the study confirmed high specificity of the determination. The test system showed good sensitivity and rapidity, allowing for the detection of as low as 0.063% (w/w) chicken meat in raw meat mixtures within 20 min. As a result of the testing of raw and cooked meat products, it was shown that the test system can reliably recognize specific immunoglobulins even after heat processing. The proposed assay can be recommended for rapid on-site screening control of the composition and quality of meat products.
本研究开发了一种免疫层析测试系统，旨在检测肉类产品中的鸡肉添加剂。该系统基于免疫球蛋白的夹心式横向流动免疫分析（LFIA）作为物种鉴定的生物标志物。基于金纳米颗粒作为抗物种抗体标记的 LFIA 用于测定鸡肉免疫球蛋白，并相应地测定食品中的鸡肉。该测定与研究中测试的哺乳动物物种无交叉反应，证实了其高特异性。该测试系统具有良好的灵敏度和快速性，可在 20 分钟内检测到生肉混合物中低至 0.063%（w/w）的鸡肉。通过对生肉和熟肉产品的测试，结果表明即使经过热处理，该测试系统也能可靠地识别特定的免疫球蛋白。该检测方法可推荐用于快速现场筛选控制肉类产品的组成和质量。
