Normandie Univ, Laboratoire SMS-EA3233, UNIROUEN, FR3038, Place Emile Blondel, F-76821, Mont-Saint-Aignan Cedex, France; SGS France laboratoire de Rouen, Technopôle du Madrillet, 65 Avenue Ettore Bugatti, Saint Etienne du Rouvray F-76801 Cedex, France.
SGS France laboratoire de Rouen, Technopôle du Madrillet, 65 Avenue Ettore Bugatti, Saint Etienne du Rouvray F-76801 Cedex, France.
Food Chem. 2021 Oct 15;359:129932. doi: 10.1016/j.foodchem.2021.129932. Epub 2021 Apr 23.
Performances of multiresidue analysis of one hundred of pesticides and contaminants, using GC-Q-Orbitrap method in full scan mode were compared to those obtained with GC-triple-quadrupole method in multiple reaction monitoring mode. In terms of sensitivity, 86% of molecules exhibited lower limit of detection values using GC-Q-Orbitrap than using GC-triple-quadrupole. For the GC-Q-Orbitrap method, more than 85% of the pesticides and contaminants showed good recovery [70-120%] in wheat samples, with relative standard deviation values < 20%. GC-Q-Orbitrap method appeared the most sensitive for most pesticides studied in wheat with limit of quantification values ranged between 0.1 µg/kg and 4 µg/kg. Moreover, the matrix effect was acceptable in wheat extracts for 84 molecules but strong suppression of the chromatographic signal was observed for 16 molecules for the GC-Q-Orbitrap method. The injection of unpurified wheat extracts spiked at 10 µg/kg proved the potential of the GC-Q-Orbitrap method for use in performing high-throughput pesticide screening.
采用全扫描模式的 GC-Q-Orbitrap 方法与采用多重反应监测模式的 GC-三重四极杆方法对一百种农药和污染物的多残留分析性能进行了比较。在灵敏度方面，使用 GC-Q-Orbitrap 检测到的 86%的分子的检测限值低于使用 GC-三重四极杆检测到的。对于 GC-Q-Orbitrap 方法，在小麦样品中，超过 85%的农药和污染物表现出良好的回收率[70-120%]，相对标准偏差值<20%。GC-Q-Orbitrap 方法对小麦中大多数研究的农药最敏感，定量限范围在 0.1μg/kg 至 4μg/kg 之间。此外，对于 84 种分子，小麦提取物中的基质效应是可以接受的，但对于 16 种分子，GC-Q-Orbitrap 方法观察到色谱信号的强烈抑制。对 10μg/kg 浓度未净化的小麦提取物进行进样，证明了 GC-Q-Orbitrap 方法在高通量农药筛选中的应用潜力。
