• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用同步辐射傅里叶变换红外显微镜、分子对接和体外研究探测硫酸化半乳糖对胆管癌细胞的抗癌活性。

Probing the Anti-Cancer Potency of Sulfated Galactans on Cholangiocarcinoma Cells Using Synchrotron FTIR Microspectroscopy, Molecular Docking, and In Vitro Studies.

机构信息

Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

出版信息

Mar Drugs. 2021 Apr 30;19(5):258. doi: 10.3390/md19050258.

DOI:10.3390/md19050258
PMID:33946151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8145517/
Abstract

Sulfated galactans (SG) isolated from red alga have been reported to inhibit the growth of cholangiocarcinoma (CCA) cells, which was similar to the epidermal growth factor receptor (EGFR)-targeted drug, cetuximab. Herein, we studied the anti-cancer potency of SG compared to cetuximab. Biological studies demonstrated SG and cetuximab had similar inhibition mechanisms in CCA cells by down-regulating EGFR/ERK pathway, and the combined treatment induced a greater inhibition effect. The molecular docking study revealed that SG binds to the dimerization domain of EGFR, and this was confirmed by dimerization assay, which showed that SG inhibited ligand-induced EGFR dimer formation. Synchrotron FTIR microspectroscopy was employed to examine alterations in cellular macromolecules after drug treatment. The SR-FTIR-MS elicited similar spectral signatures of SG and cetuximab, pointing towards the bands of RNA/DNA, lipids, and amide I vibrations, which were inconsistent with the changes of signaling proteins in CCA cells after drug treatment. Thus, this study demonstrates the underlined anti-cancer mechanism of SG by interfering with EGFR dimerization. In addition, we reveal that FTIR signature spectra offer a useful tool for screening anti-cancer drugs' effect.

摘要

硫酸半乳聚糖（SG）从红藻中分离出来，据报道能抑制胆管癌（CCA）细胞的生长，这与表皮生长因子受体（EGFR）靶向药物西妥昔单抗相似。在此，我们研究了 SG 与西妥昔单抗相比的抗癌效力。生物学研究表明，SG 和西妥昔单抗通过下调 EGFR/ERK 通路在 CCA 细胞中有相似的抑制机制，联合治疗诱导了更大的抑制作用。分子对接研究表明 SG 结合到 EGFR 的二聚化结构域，这通过二聚化实验得到证实，该实验表明 SG 抑制配体诱导的 EGFR 二聚体形成。同步辐射傅里叶变换红外显微镜（SR-FTIR）用于检查药物处理后细胞大分子的变化。SR-FTIR-MS 产生了与 SG 和西妥昔单抗相似的光谱特征，指向 RNA/DNA、脂质和酰胺 I 振动带，这与 CCA 细胞在药物处理后信号蛋白的变化不一致。因此，本研究通过干扰 EGFR 二聚化证明了 SG 的潜在抗癌机制。此外，我们揭示了傅里叶变换红外光谱特征提供了一种用于筛选抗癌药物效果的有用工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/5154235ca4e9/marinedrugs-19-00258-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/2fda242e71e0/marinedrugs-19-00258-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/91dbfd7c3cc9/marinedrugs-19-00258-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/5cdd83f11e03/marinedrugs-19-00258-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/0dc21fc0d1f1/marinedrugs-19-00258-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/13c1677ff1d3/marinedrugs-19-00258-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/14cea7991ff6/marinedrugs-19-00258-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/4698f942d51a/marinedrugs-19-00258-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/5154235ca4e9/marinedrugs-19-00258-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/2fda242e71e0/marinedrugs-19-00258-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/91dbfd7c3cc9/marinedrugs-19-00258-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/5cdd83f11e03/marinedrugs-19-00258-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/0dc21fc0d1f1/marinedrugs-19-00258-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/13c1677ff1d3/marinedrugs-19-00258-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/14cea7991ff6/marinedrugs-19-00258-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/4698f942d51a/marinedrugs-19-00258-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d0/8145517/5154235ca4e9/marinedrugs-19-00258-g008.jpg

相似文献

1
Probing the Anti-Cancer Potency of Sulfated Galactans on Cholangiocarcinoma Cells Using Synchrotron FTIR Microspectroscopy, Molecular Docking, and In Vitro Studies.利用同步辐射傅里叶变换红外显微镜、分子对接和体外研究探测硫酸化半乳糖对胆管癌细胞的抗癌活性。
Mar Drugs. 2021 Apr 30;19(5):258. doi: 10.3390/md19050258.
2
Sulfated galactans from the red seaweed Gracilaria fisheri exerts anti-migration effect on cholangiocarcinoma cells.来自红藻费氏江蓠的硫酸化半乳聚糖对胆管癌细胞具有抗迁移作用。
Phytomedicine. 2017 Dec 1;36:59-67. doi: 10.1016/j.phymed.2017.09.014. Epub 2017 Sep 28.
3
Sulfated Galactans from Red Seaweed Gracilaria fisheri Target EGFR and Inhibit Cholangiocarcinoma Cell Proliferation.红海巨藻硫酸半乳聚糖靶向 EGFR 并抑制胆管癌细胞增殖。
Am J Chin Med. 2017;45(3):615-633. doi: 10.1142/S0192415X17500367. Epub 2017 Apr 6.
4
Synchrotron FTIR microspectroscopy revealed apoptosis-induced biomolecular changes of cholangiocarcinoma cells treated with ursolic acid.同步辐射傅里叶变换红外显微镜光谱分析揭示了熊果酸处理诱导的胆管癌细胞凋亡的生物分子变化。
Biochim Biophys Acta Gen Subj. 2020 Dec;1864(12):129708. doi: 10.1016/j.bbagen.2020.129708. Epub 2020 Aug 15.
5
Monitoring the Progression of Liver Fluke-Induced Cholangiocarcinoma in a Hamster Model Using Synchrotron FTIR Microspectroscopy and Focal Plane Array Infrared Imaging.应用同步辐射傅里叶变换红外显微镜和焦平面阵列红外成像技术监测仓鼠模型肝吸虫诱导的胆管癌的进展。
Anal Chem. 2020 Dec 1;92(23):15361-15369. doi: 10.1021/acs.analchem.0c02656. Epub 2020 Nov 10.
6
Molecular mechanism of Spatholobi Caulis treatment for cholangiocarcinoma based on network pharmacology, molecular docking, and molecular dynamics simulation.基于网络药理学、分子对接和分子动力学模拟探讨鸡血藤治疗胆管癌的分子机制。
Naunyn Schmiedebergs Arch Pharmacol. 2024 Aug;397(8):5789-5806. doi: 10.1007/s00210-024-02985-0. Epub 2024 Feb 7.
7
Classification of Gemcitabine resistant Cholangiocarcinoma cell lines using synchrotron FTIR microspectroscopy.使用同步辐射傅里叶变换红外光谱法对吉西他滨耐药胆管癌细胞系进行分类
J Biophotonics. 2017 Mar;10(3):367-376. doi: 10.1002/jbio.201500253. Epub 2016 Mar 21.
8
Sulfated galactans from Gracilaria fisheri bind to shrimp haemocyte membrane proteins and stimulate the expression of immune genes.费氏江蓠中的硫酸化半乳聚糖与对虾血细胞膜蛋白结合并刺激免疫基因的表达。
Fish Shellfish Immunol. 2015 Nov;47(1):231-8. doi: 10.1016/j.fsi.2015.09.006. Epub 2015 Sep 9.
9
Immunostimulatory activity of sulfated galactans isolated from the red seaweed Gracilaria fisheri and development of resistance against white spot syndrome virus (WSSV) in shrimp.从红海草 Gracilaria fisheri 中分离得到的硫酸化半乳糖聚糖的免疫刺激活性及对虾白斑综合征病毒（WSSV）的抗性发展。
Fish Shellfish Immunol. 2014 Jan;36(1):52-60. doi: 10.1016/j.fsi.2013.10.010. Epub 2013 Oct 23.
10
Induction of apoptotic cell death of cholangiocarcinoma cells by tiliacorinine from Tiliacora triandra: A mechanistic insight.三白草科三叶苦诱导胆管癌细胞凋亡的机制研究。
Biochim Biophys Acta Gen Subj. 2023 Dec;1867(12):130486. doi: 10.1016/j.bbagen.2023.130486. Epub 2023 Oct 7.

引用本文的文献

1
Sulfated Galactans from Agarophytes: Review of Extraction Methods, Structural Features, and Biological Activities.琼胶来源硫酸半乳聚糖：提取方法、结构特征和生物活性综述。
Biomolecules. 2023 Dec 5;13(12):1745. doi: 10.3390/biom13121745.
2
Marine Migrastatics: A Comprehensive 2022 Update.海洋洄游生物：2022 年全面更新。
Mar Drugs. 2022 Apr 19;20(5):273. doi: 10.3390/md20050273.

本文引用的文献

1
Fourier Transform Infrared Spectroscopy in Oral Cancer Diagnosis.傅里叶变换红外光谱技术在口腔癌诊断中的应用。
Int J Mol Sci. 2021 Jan 26;22(3):1206. doi: 10.3390/ijms22031206.
2
Monitoring the Progression of Liver Fluke-Induced Cholangiocarcinoma in a Hamster Model Using Synchrotron FTIR Microspectroscopy and Focal Plane Array Infrared Imaging.应用同步辐射傅里叶变换红外显微镜和焦平面阵列红外成像技术监测仓鼠模型肝吸虫诱导的胆管癌的进展。
Anal Chem. 2020 Dec 1;92(23):15361-15369. doi: 10.1021/acs.analchem.0c02656. Epub 2020 Nov 10.
3
Synchrotron FTIR microspectroscopy revealed apoptosis-induced biomolecular changes of cholangiocarcinoma cells treated with ursolic acid.
同步辐射傅里叶变换红外显微镜光谱分析揭示了熊果酸处理诱导的胆管癌细胞凋亡的生物分子变化。
Biochim Biophys Acta Gen Subj. 2020 Dec;1864(12):129708. doi: 10.1016/j.bbagen.2020.129708. Epub 2020 Aug 15.
4
FTIR spectra signatures reveal different cellular effects of EGFR inhibitors on nonsmall cell lung cancer cells.FTIR 光谱特征揭示了表皮生长因子受体抑制剂对非小细胞肺癌细胞的不同细胞效应。
J Biophotonics. 2020 Mar;13(3):e201960012. doi: 10.1002/jbio.201960012. Epub 2020 Jan 8.
5
Ligand binding effects on the activation of the EGFR extracellular domain.配体结合对 EGFR 细胞外域激活的影响。
Phys Chem Chem Phys. 2019 Apr 10;21(15):8141-8151. doi: 10.1039/c8cp07496h.
6
Cholangiocarcinoma Trends, Incidence, and Relative Survival in Khon Kaen, Thailand From 1989 Through 2013: A Population-Based Cancer Registry Study.泰国孔敬府胆管癌的趋势、发病率和相对生存率：1989 年至 2013 年的一项基于人群的癌症登记研究。
J Epidemiol. 2019 May 5;29(5):197-204. doi: 10.2188/jea.JE20180007. Epub 2018 Aug 4.
7
Sulfated galactans from the red seaweed Gracilaria fisheri exerts anti-migration effect on cholangiocarcinoma cells.来自红藻费氏江蓠的硫酸化半乳聚糖对胆管癌细胞具有抗迁移作用。
Phytomedicine. 2017 Dec 1;36:59-67. doi: 10.1016/j.phymed.2017.09.014. Epub 2017 Sep 28.
8
Phellinus linteus Grown on Germinated Brown Rice Increases Cetuximab Sensitivity of KRAS-Mutated Colon Cancer.在发芽糙米上生长的桑黄可提高KRAS突变型结肠癌对西妥昔单抗的敏感性。
Int J Mol Sci. 2017 Aug 11;18(8):1746. doi: 10.3390/ijms18081746.
9
Real-time Characterization of Antibody Binding to Receptors on Living Immune Cells.活免疫细胞上抗体与受体结合的实时表征
Front Immunol. 2017 Apr 24;8:455. doi: 10.3389/fimmu.2017.00455. eCollection 2017.
10
Sulfated Galactans from Red Seaweed Gracilaria fisheri Target EGFR and Inhibit Cholangiocarcinoma Cell Proliferation.红海巨藻硫酸半乳聚糖靶向 EGFR 并抑制胆管癌细胞增殖。
Am J Chin Med. 2017;45(3):615-633. doi: 10.1142/S0192415X17500367. Epub 2017 Apr 6.