Park Jin-Ho, Park Su A, Kang Young-Hoon, Hwa So Myeong, Koh Eun-Byeol, Hwang Sun-Chul, Oh Se Heang, Byun June-Ho
Department of Oral and Maxillofacial Surgery, Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju 52727, Korea.
Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Korea.
Life (Basel). 2021 Apr 30;11(5):410. doi: 10.3390/life11050410.
Coupling between osteoblast-mediated bone formation and osteoclast-mediated bone resorption maintains both mechanical integrity and mineral homeostasis. Zinc is required for the formation, mineralization, growth, and maintenance of bones. We examined the effects of zinc sulfate on osteoblastic differentiation of human periosteum-derived cells (hPDCs) and osteoclastic differentiation of THP-1 cells. Zinc sulfate enhanced the osteoblastic differentiation of hPDCs; however, it did not affect the osteoclastic differentiation of THP-1 cells. The levels of extracellular signaling-related kinase (ERK) were strongly increased during osteoblastic differentiation in zinc sulfate-treated hPDCs, compared with other mitogen-activated protein kinases (MAPKs). Zinc sulfate also promoted osteogenesis in hPDCs and THP-1 cells co-cultured with the ratio of one osteoclast to one osteoblast, as indicated by alkaline phosphatase levels, mineralization, and cellular calcium contents. In addition, the receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio was decreased in the zinc sulfate-treated co-cultures. Our results suggest that zinc sulfate enhances osteogenesis directly by promoting osteoblastic differentiation and osteogenic activities in osteoblasts and indirectly by inhibiting osteoclastic bone resorption through a reduced RANKL/OPG ratio in co-cultured osteoblasts and osteoclasts.
成骨细胞介导的骨形成与破骨细胞介导的骨吸收之间的偶联维持了机械完整性和矿物质稳态。锌是骨骼形成、矿化、生长和维持所必需的。我们研究了硫酸锌对人骨膜来源细胞（hPDCs）成骨细胞分化和THP-1细胞破骨细胞分化的影响。硫酸锌增强了hPDCs的成骨细胞分化；然而，它对THP-1细胞的破骨细胞分化没有影响。与其他丝裂原活化蛋白激酶（MAPKs）相比，在硫酸锌处理的hPDCs成骨细胞分化过程中，细胞外信号调节激酶（ERK）水平显著升高。硫酸锌还促进了以1个破骨细胞与1个成骨细胞比例共培养的hPDCs和THP-1细胞的成骨作用，这通过碱性磷酸酶水平、矿化和细胞钙含量得以体现。此外，在硫酸锌处理的共培养物中，核因子κB受体活化因子配体（RANKL）/骨保护素（OPG）比值降低。我们的结果表明，硫酸锌通过促进成骨细胞的成骨细胞分化和成骨活性直接增强成骨作用，并通过降低共培养的成骨细胞和破骨细胞中的RANKL/OPG比值间接抑制破骨细胞骨吸收。
