Blanco Vázquez Cristina, Barral Thiago Doria, Romero Beatriz, Queipo Manuel, Merediz Isabel, Quirós Pablo, Armenteros José Ángel, Juste Ramón, Domínguez Lucas, Domínguez Mercedes, Casais Rosa, Balseiro Ana
Servicio Regional de Investigación y Desarrollo Agroalimentario del Principado de Asturias (SERIDA), 33300 Villaviciosa, Spain.
Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia, 40.110-100 Salvador, Bahia, Brazil.
Animals (Basel). 2021 Apr 30;11(5):1294. doi: 10.3390/ani11051294.
The present work investigated the prevalence, spatial distribution, and temporal distribution of tuberculosis (TB) in free-ranging Eurasian badgers () and cattle in Asturias (Atlantic Spain) during a 13-year follow-up. The study objective was to assess the role of badgers as a TB reservoir for cattle and other sympatric wild species in the region. Between 2008 and 2020, 673 badgers (98 trapped and 575 killed in road traffic accidents) in Asturias were necropsied, and their tissue samples were cultured for the complex (MTC) isolation. Serum samples were tested in an in-house indirect P22 ELISA to detect antibodies against the MTC. In parallel, data on MTC isolation and single intradermal tuberculin test results were extracted for cattle that were tested and culled as part of the Spanish National Program for the Eradication of Bovine TB. A total of 27/639 badgers (4.23%) were positive for MTC based on bacterial isolation, while 160/673 badgers (23.77%) were found to be positive with the P22 ELISA. The rate of seropositivity was higher among adult badgers than subadults. Badger TB status was spatially and temporally associated with cattle TB status. Our results cannot determine the direction of possible interspecies transmission, but they are consistent with the idea that the two hosts may exert infection pressure on each other. This study highlights the importance of the wildlife monitoring of infection and disease during epidemiological interventions in order to optimize outcomes.
本研究在13年的随访期间，调查了西班牙阿斯图里亚斯地区（大西洋沿岸）自由放养的欧亚獾和牛结核病（TB）的流行情况、空间分布和时间分布。研究目的是评估獾作为该地区牛和其他同域野生物种结核病储存宿主的作用。2008年至2020年期间，对阿斯图里亚斯地区的673只獾（98只诱捕，575只因道路交通事故死亡）进行了尸检，并对其组织样本进行培养以分离结核分枝杆菌复合群（MTC）。血清样本采用内部间接P22 ELISA检测，以检测抗MTC抗体。同时，提取了作为西班牙国家牛结核病根除计划一部分接受检测和扑杀的牛的MTC分离数据和单次皮内结核菌素试验结果。基于细菌分离，共有27/639只獾（4.23%）MTC呈阳性，而160/673只獾（23.77%）P22 ELISA检测呈阳性。成年獾的血清阳性率高于亚成年獾。獾的结核病状况在空间和时间上与牛的结核病状况相关。我们的结果无法确定可能的种间传播方向，但与两种宿主可能相互施加感染压力的观点一致。这项研究强调了在流行病学干预期间对野生动物感染和疾病进行监测以优化结果的重要性。
