Veterinary Epidemiology Unit, Department of Agriculture, Environment and Rural Affairs, Belfast BT4 3SB, Northern Ireland, UK.
Veterinary Epidemiology Unit, Department of Agriculture, Environment and Rural Affairs, Belfast BT4 3SB, Northern Ireland, UK.
Res Vet Sci. 2020 Jun;130:170-178. doi: 10.1016/j.rvsc.2020.03.007. Epub 2020 Mar 3.
European badgers (Meles meles) are accepted as a wildlife reservoir host for Mycobacterium bovis, which causes bovine tuberculosis (bTB) in the British Isles. The objective of this study was to evaluate the use of Dual Path Platform (DPP) VetTB test (Chembio Diagnostic Systems Inc., Medford, NY, USA) within a Test and Vaccinate or Remove (TVR) wildlife research intervention project. Blood samples were collected from 456 individual badgers, trapped in 2015 and 2016, and tested in the field with DPP VetTB test using whole blood. Additionally, whole blood and serum samples were taken to the laboratory for further DPP VetTB testing and for gamma interferon (IFN-γ) testing. Swabs were taken from the oropharynx and trachea and submitted for bacteriological culture as were swabs from wounds, if present. Field DPP VetTB test positive badgers were euthanised and underwent post-mortem examination and bTB confirmatory testing. The results demonstrated that the test performed as well in the field using whole blood as DPP Vet TB tests in the laboratory using sera or whole blood, and as well as other established tests for M. bovis. Visual assessment of the DPP VetTB test using serum under laboratory conditions showed a high degree of consistency between raters. Using a relative gold standard (parallel interpretation of IFN-γ assay and oropharyngeal/tracheal sample/culture), sensitivity estimates for the DPP VetTB test using sera and whole blood were 0.5 (95%CI 0.34-0.66) and 0.42 (95%CI 0.24-0.66), respectively. Specificity estimates were 0.95 (95%CI 0.93-0.97) for sera and 0.89 (95%CI 0.86-0.92) for whole blood. Parallel interpretation of Band 1 (MPB83) and Band 2 (CFP-10/ESAT-6) of the DPP VetTB test was not superior to interpretation of Band 1 only. The results give confidence in the reliability and reproducibility of the DPP VetTB test for badgers under field conditions and therefore it is considered appropriate for use in a badger bTB control campaign.
欧洲獾（Meles meles）被认为是牛分枝杆菌（Mycobacterium bovis）的野生动物储主，该细菌会导致不列颠群岛的牛结核病（bTB）。本研究的目的是评估 Dual Path Platform（DPP） VetTB 测试（Chembio Diagnostic Systems Inc.，Medford，NY，USA）在 Test and Vaccinate or Remove（TVR）野生动物研究干预项目中的应用。2015 年和 2016 年，从 456 只被捕獾的个体中采集血液样本，并在现场使用 DPP VetTB 测试进行检测，该测试使用全血。此外，还采集全血和血清样本送往实验室进行进一步的 DPP VetTB 测试和γ干扰素（IFN-γ）测试。采集口咽和气管拭子进行细菌培养，如果存在伤口，也采集拭子进行培养。现场 DPP VetTB 测试阳性的獾被安乐死，并进行剖检和牛分枝杆菌确认性测试。结果表明，现场使用全血进行 DPP VetTB 测试的效果与实验室使用血清或全血进行 DPP Vet TB 测试的效果一样好，与其他牛分枝杆菌检测方法的效果也一样好。在实验室条件下使用血清对 DPP VetTB 测试进行视觉评估，结果表明评估者之间具有高度一致性。使用相对金标准（IFN-γ 检测平行解释和口咽/气管样本/培养），DPP VetTB 测试使用血清和全血的灵敏度估计值分别为 0.5（95%CI 0.34-0.66）和 0.42（95%CI 0.24-0.66）。血清的特异性估计值为 0.95（95%CI 0.93-0.97），全血的特异性估计值为 0.89（95%CI 0.86-0.92）。DPP VetTB 测试的 Band 1（MPB83）和 Band 2（CFP-10/ESAT-6）平行解释并不优于仅解释 Band 1。这些结果使人们对 DPP VetTB 测试在野外条件下对獾的可靠性和可重复性有信心，因此认为它适合用于獾牛结核病控制活动。
