Bio-Insecticide of and Extract from Cell Suspensions and Their Inhibitory Effect against Serine, Cysteine, and Metalloproteinases of the Red Palm Weevil ().

The current study was designed to investigate the insecticide role of volatile constituents produced from cell suspensions of and against . Constituents were extracted from cell suspension after 40 days. Growth kinetics were measured with an inoculation of and identified by GC-MS. Total volatile phenolic constituents were measured. Insecticidal activity against (adult) and proteolytic enzyme activity in larvae were assessed. GC-MS showed that the extract has higher amounts of thymol, -cymene, γ-terpinene, β-caryophyllene, and linalool in comparison to the extract, which is rich in estragole, β-terpineol, ()-β-ocimene, 1,8-cineole, germacrene D, and eugenol. The extract showed an LC of 1032 µg/mL, followed by with an LC of 1246 µg/mL. The IC values against the total proteases were 110.8 and 119.4 µg/mL for   and  , respectively. The IC for the trypsin-like serine proteinase assessment was 81.6 and 91 µg/mL for   and  , respectively. Cysteine, chymotrypsin, and metalloproteinase assessment showed an IC above 5000 µg/mL for both extracts. The study is proposed as a potential approach to use and extract as a bio-insecticide against using an accessible and efficient cell suspension technique.