一种跟踪和评估蓖麻毒素对人源双链DNA动力学催化作用的电化学方法。

An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA.

作者信息

Oliveira George, Schneedorf José Maurício

机构信息

Department of Biochemistry, Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas 37130-000, Brazil.

出版信息

Life (Basel). 2021 Apr 29;11(5):405. doi: 10.3390/life11050405.

DOI:10.3390/life11050405

PMID:33946642

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8145570/

Abstract

International authorities classify the ricin toxin, present in castor seeds, as a potential agent for use in bioterrorism. Therefore, the detection, identification, and characterization of ricin are considered the first actions for its risk assessment during a suspected exposure, parallel to the development of therapeutic and medical countermeasures. In this study, we report the kinetic analysis of electro-oxidation of adenine released from hsDNA by the catalytic action of ricin by square wave voltammetry. The results suggest that ricin-mediated adenine release exhibited an unusual kinetic profile, with a progress curve controlled by the accumulation of the product and the values of the kinetic constants of 46.6 µM for and 2000 min for , leading to a catalytic efficiency of 7.1 × 10 s M.

摘要

国际权威机构将蓖麻籽中存在的蓖麻毒素列为可用于生物恐怖主义的潜在制剂。因此，蓖麻毒素的检测、鉴定和特性分析被视为在疑似接触期间对其进行风险评估的首要行动，这与治疗和医学应对措施的开发并行。在本研究中，我们报告了通过方波伏安法对蓖麻毒素催化作用下从热变性DNA（hsDNA）释放的腺嘌呤进行电氧化的动力学分析。结果表明，蓖麻毒素介导的腺嘌呤释放呈现出异常的动力学特征，其进程曲线受产物积累控制，动力学常数的值分别为46.6 μM和2000 min，催化效率为7.1×10 s M。