Ito Masaaki, Toriumi Taku, Hiratsuka Takahiro, Imura Hideto, Akiyama Yasunori, Chimedtseren Ichinnorov, Arai Yoshinori, Yamaguchi Kazuhiro, Azuma Akihiko, Hata Ken-Ichiro, Natsume Nagato, Honda Masaki
Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan.
Department of Oral Anatomy, School of Dentistry, Aichi Gakuin University, Aichi 464-8650, Japan.
Materials (Basel). 2021 Apr 29;14(9):2306. doi: 10.3390/ma14092306.
This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in human alveolar cleft. Low- (group 1), medium- (group 2), and high- (group 3) cross-linked RCP particles were prepared by altering the duration of the heat-dependent dehydration reaction. Rat palatine fissures ( = 45), analogous to human congenital bone defects, were examined to evaluate the potential of bone formation by the three different RCP particles. Microcomputed tomography images were obtained to measure bone volume and bone mineral density at 4, 8, 12, and 16 weeks post grafting. Specimens were obtained for histological analysis at 16 weeks after grafting. Additionally, alkaline phosphatase and tartrate acid phosphatase staining were performed to visualize the presence of osteoblasts and osteoclasts. At 16 weeks, bone volume, bone mineral density, and new bone area measurements in group 2 were significantly higher than in any other group. In addition, the number of osteoblasts and osteoclasts on the new bone surface in group 2 was significantly higher than in any other group. Our results demonstrated that medium cross-linking was more suitable for bone formation-and could be useful in human alveolar cleft repairs as well.
本研究旨在检测基于人I型胶原蛋白的重组肽（RCP）颗粒的最佳交联密度，用于人类牙槽嵴裂的骨重建。通过改变热依赖性脱水反应的持续时间，制备了低交联（第1组）、中交联（第2组）和高交联（第3组）的RCP颗粒。检测大鼠腭裂（n = 45），其类似于人类先天性骨缺损，以评估三种不同RCP颗粒的骨形成潜力。在移植后4、8、12和16周获取微型计算机断层扫描图像，以测量骨体积和骨密度。在移植后16周获取标本进行组织学分析。此外，进行碱性磷酸酶和酒石酸磷酸酶染色，以观察成骨细胞和破骨细胞的存在情况。在16周时，第2组的骨体积、骨密度和新骨面积测量值显著高于其他任何组。此外，第2组新骨表面的成骨细胞和破骨细胞数量显著高于其他任何组。我们的结果表明，中等交联更适合骨形成，并且在人类牙槽嵴裂修复中也可能有用。
