Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea.
Nakdonggang National Institute of Biological Resources, 137, Donam 2-gil, Sangju-si 37242, Korea.
Biomolecules. 2021 Apr 29;11(5):658. doi: 10.3390/biom11050658.
We examined the effects of two histone acetyltransferase (HAT) inhibitors on the activity of rice serotonin -acetyltransferases (SNAT). Two rice recombinant SNAT isoenzymes (SNAT1 and SNAT2) were incubated in the presence of either MG149 or MB3, HAT inhibitors. MG149 significantly inhibited the SNAT enzymes in a dose-dependent manner, especially SNAT1, while SNAT2 was moderately inhibited. By contrast, MB3 had no effect on SNAT1 or SNAT2. The application of 100 μM MG149 to rice seedlings decreased melatonin by 1.6-fold compared to the control, whereas MB3 treatment did not alter the melatonin level. MG149 significantly decreased both melatonin and -acetylserotonin when rice seedlings were challenged with cadmium, a potent elicitor of melatonin synthesis in rice. Although MG149 inhibited melatonin synthesis in rice seedlings, no melatonin deficiency-induced lamina angle decrease was observed due to the insufficient suppression of SNAT2, which is responsible for the lamina angle decrease in rice.
我们研究了两种组蛋白乙酰转移酶（HAT）抑制剂对水稻 5-羟色胺乙酰转移酶（SNAT）活性的影响。在存在 HAT 抑制剂 MG149 或 MB3 的情况下，孵育两种水稻重组 SNAT 同工酶（SNAT1 和 SNAT2）。MG149 以剂量依赖性方式显著抑制 SNAT 酶，尤其是 SNAT1，而 SNAT2 则被适度抑制。相比之下，MB3 对 SNAT1 或 SNAT2 没有影响。与对照相比，100 μM MG149 处理水稻幼苗使褪黑素减少 1.6 倍，而 MB3 处理并未改变褪黑素水平。MG149 显著降低了水稻幼苗在受到镉（一种在水稻中强烈诱导褪黑素合成的有效诱导剂）挑战时的褪黑素和 -乙酰色氨酸。尽管 MG149 抑制了水稻幼苗中的褪黑素合成，但由于负责水稻叶片角度减小的 SNAT2 抑制不足，没有观察到褪黑素缺乏诱导的叶片角度减小。
