Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.
Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
ChemSusChem. 2021 Oct 5;14(19):4115-4122. doi: 10.1002/cssc.202100740. Epub 2021 Jun 1.
The research on polyethylene terephthalate (PET) hydrolyzing enzymes started in 2005; several studies are now nearing the objective of their application in biorecycling of PET, which is an urgent environmental issue. The thermostability of PET hydrolases must be higher than 70 °C, which has already been established by several thermophilic cutinases, as higher thermostability results in higher activity. Additionally, pretreatment of waste PET to more enzyme-attackable forms is necessary for PET biorecycling. This Minireview summarizes research on enzymatic PET hydrolysis from two viewpoints: 1) improvement of PET hydrolases by focusing on their thermostabilities by mutation of enzyme genes, their expression in several hosts, and their modifications; and 2) processing of waste PET to readily biodegradable forms. Finally, the outlook of PET biorecycling is described.
聚对苯二甲酸乙二醇酯（PET）水解酶的研究始于 2005 年；目前，几项研究已接近将其应用于 PET 的生物回收的目标，这是一个紧迫的环境问题。PET 水解酶的热稳定性必须高于 70°C，这已经被几种嗜热角质酶所证实，因为较高的热稳定性会导致更高的活性。此外，为了进行 PET 的生物回收，需要对废 PET 进行预处理，使其转化为更易于酶攻击的形式。本综述从两个方面总结了酶法 PET 水解的研究进展：1）通过突变酶基因、在多种宿主中表达以及修饰来提高 PET 水解酶的热稳定性；2）将废 PET 加工成易于生物降解的形式。最后，描述了 PET 的生物回收前景。
