D-葡萄糖对新生小牛空肠和兔回肠体外短路电流的影响。

Effect of D-glucose on in vitro short-circuit current in neonatal calf jejunum and rabbit ileum.

作者信息

Ferrante P L, Freeman D E, Whitlock R H, Kronfeld D S

机构信息

Department of Clinical Studies, University of Pennsylvania, School of Veterinary Medicine, Kennett Square 19348.

出版信息

Am J Vet Res. 1988 May;49(5):715-9.

PMID:3395017

Abstract

Thin sheets of mucosa from small intestine of neonatal calves were mounted in incubation chambers for in vitro studies. These mucosal sheets generated a potential difference (PD) of 2.05 +/- 0.02 mV (mean +/- SEM), short-circuit current (SCC) of 23.32 +/- 3.81 microA x cm2, and tissue resistance of 86.22 +/- 4.41 ohms x cm2 (n = 6). Ouabain in the serosal bathing solution caused a sharp decrease in the SCC (P less than 0.01) and PD (P less than 0.005), a decrease in tissue K content (P less than 0.05), and an increase in tissue Na content (P less than 0.05). The mucosa responded to D-glucose by an increase in PD (P less than 0.001) and SCC (P less than 0.001). In vitro methods used in the calf were validated in similar experiments on rabbit ileum.

摘要

将新生小牛小肠的薄黏膜片安装在培养室中进行体外研究。这些黏膜片产生的电位差（PD）为2.05±0.02 mV（平均值±标准误），短路电流（SCC）为23.32±3.81 μA×cm²，组织电阻为86.22±4.41欧姆×cm²（n = 6）。浆膜浴液中的哇巴因导致SCC急剧下降（P<0.01）和PD下降（P<0.005），组织钾含量降低（P<0.05），组织钠含量增加（P<0.05）。黏膜对D-葡萄糖的反应是PD增加（P<0.001）和SCC增加（P<0.001）。在小牛身上使用的体外方法在兔回肠的类似实验中得到了验证。