Lab of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.
Instituto Nacional de Biomarcadores em Neuropsiquiatria (INBION), Conselho Nacional de Desenvolvimento Científico e Tecnológico, São Paulo, Brazil.
Methods Mol Biol. 2021;2228:341-352. doi: 10.1007/978-1-0716-1024-4_24.
Here, we describe a proteomic pipeline to use a human microglial cell line as a biological model to study schizophrenia. In order to maximize the proteome coverage, we apply two-dimensional liquid chromatography coupled with ultra-definition MS mass spectrometry (LC-UDMS) using a data-independent acquisition (DIA) approach, with an optimization of drift time collision energy.
在这里，我们描述了一个蛋白质组学研究方案，使用人类小胶质细胞系作为生物模型来研究精神分裂症。为了最大限度地提高蛋白质组覆盖范围，我们应用二维液相色谱与超分辨率 MS 质谱（LC-UDMS）联用，采用数据非依赖性采集（DIA）方法，并优化了漂移时间碰撞能量。
