Souza Gustavo Henrique Martins Ferreira, Guest Paul C, Martins-de-Souza Daniel
Mass Spectrometry Applications & Development Laboratory, Waters Corporation, 125, Alphaville Industrial, Barueri, 06455-020, Campinas, São Paulo, SP, Brazil.
Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Methods Mol Biol. 2017;1546:57-73. doi: 10.1007/978-1-4939-6730-8_4.
Proteomic tools can only be implemented in clinical settings if high-throughput, automated, sensitive, and accurate methods are developed. This has driven researchers to the edge of mass spectrometry (MS)-based proteomics capacity. Here we provide an overview of recent achievements in mass spectrometric technologies and instruments. This includes development of high and ultra definition-MS (HDMS and UDMS) through implementation of ion mobility (IM) MS towards sensitive and accurate label-free proteomics using ultra performance liquid chromatography (UPLC). Label free UPLC-HDMS is less expensive than labeled-based quantitative proteomics and has no limits regarding the number of samples that can be analyzed and compared, which is an important requirement for supporting clinical applications.
只有开发出高通量、自动化、灵敏且准确的方法，蛋白质组学工具才能在临床环境中得以应用。这促使研究人员迈向基于质谱（MS）的蛋白质组学能力的极限。在此，我们概述了质谱技术和仪器方面的最新成果。这包括通过将离子淌度（IM）质谱与超高效液相色谱（UPLC）相结合，用于灵敏且准确的无标记蛋白质组学，从而开发出高分辨和超高分辨质谱（HDMS和UDMS）。无标记的UPLC-HDMS比基于标记的定量蛋白质组学成本更低，并且在可分析和比较的样本数量方面没有限制，这是支持临床应用的一项重要要求。
