Pappolla M A
Laboratory Service, Montrose Veterans Administration Medical Center, NY 10548.
Arch Pathol Lab Med. 1988 Aug;112(8):787-90.
A simple technique using monochromatic light was applied to computer-assisted image analysis of tissue sections. The method consists of the determination of the spectral characteristics of different elements of interest and selective monochromatic illumination of a microscopic field followed by image analysis. Results show a dramatic increase in resolution and contrast. By means of an image subtraction technique at two wavelengths, it was possible to achieve specific optical isolation and quantification of image components.
一种使用单色光的简单技术被应用于组织切片的计算机辅助图像分析。该方法包括确定不同感兴趣元素的光谱特征，对微观视野进行选择性单色照明，然后进行图像分析。结果显示分辨率和对比度显著提高。通过在两个波长处的图像减法技术，可以实现图像成分的特定光学分离和定量。
