Taper H S, Somer M P, Lans M, de Gerlache J, Roberfroid M
Laboratory of Toxicological and Cancerological Biochemistry, University of Louvain, Bruxelles, Belgium.
Arch Toxicol. 1988 Apr;61(5):406-10. doi: 10.1007/BF00334622.
A histochemical technique for detection of the in vivo induced cellular aldehydes based on the direct Schiff's reaction is reported in this paper. CCl4-intoxicated rat liver was used as an experimental model. Fresh and non-pretreated rat liver cryostat sections fixed in 10% formol calcium solution and washed in distilled water were exposed to Schiff's reagent. The sections were then immersed in two baths of sodium bisulphite solution, then in water, dehydrated in ethanol, cleared in xylene and mounted in a synthetic anhydrous mounting medium. As Schiff positive areas presented well circumscribed foci which increased with time following intoxication, semi-quantitative planimetric measurements were feasible. The direct Schiff's reaction detects cellular aldehydes in a sensitive, rapid, histologically and topographically estimable way. The appearance of these aldehydes precedes distinctly morphological alterations detectable by other histochemical or histological techniques. No positive results were obtained in control, non-intoxicated rat livers. Inhibition of this direct Schiff's reaction was obtained in positive control rat liver sections preincubated in solutions of aldehyde blockers. Histochemical detection of aldehydes may give useful information on different aspects of tissue and organ intoxication such as their topography, appearance, evolution, extension, consequences and effects of treatment. The direct Schiff's reaction can be considered as a valuable tool in fundamental and applied research dealing with various toxicological, environmental, pathological, cancer-related and therapeutic problems.
本文报道了一种基于直接席夫反应检测体内诱导细胞醛类的组织化学技术。将四氯化碳中毒的大鼠肝脏用作实验模型。将新鲜且未经预处理的大鼠肝脏低温切片固定于10%甲醛钙溶液中，并用蒸馏水冲洗，然后将其置于席夫试剂中。切片随后浸入两浴亚硫酸氢钠溶液中，再放入水中，用乙醇脱水，用二甲苯透明，并封固于合成无水封固介质中。由于席夫阳性区域呈现界限分明的病灶，且随着中毒后时间的延长而增加，因此半定量平面测量是可行的。直接席夫反应以一种敏感、快速、在组织学和拓扑学上可评估的方式检测细胞醛类。这些醛类的出现明显先于其他组织化学或组织学技术可检测到的形态学改变。在对照的未中毒大鼠肝脏中未获得阳性结果。在醛类阻滞剂溶液中预孵育的阳性对照大鼠肝脏切片中，该直接席夫反应受到抑制。醛类的组织化学检测可提供有关组织和器官中毒不同方面的有用信息，例如其拓扑结构、外观、演变、范围、后果及治疗效果。直接席夫反应可被视为在处理各种毒理学、环境、病理学、癌症相关及治疗问题的基础研究和应用研究中的一种有价值的工具。
