Benedetti A, Malvaldi G, Fulceri R, Comporti M
Cancer Res. 1984 Dec;44(12 Pt 1):5712-7.
Since it is known that tumor cell membranes have lost the capacity to undergo lipid peroxidation, it seemed of interest to investigate whether the loss of susceptibility to lipid peroxidation represents a tumoral marker appearing in preneoplastic cells together with the other known tumoral markers. A histochemical technique was developed to detect lipid peroxidation in individual cells of liver sections exposed to effective prooxidants. The technique was based on the detection of protein-bound aldehydes (alkenals) with the use of the Schiff's reagent. The latter reagent can also detect carbonyl function present in acyl residues of peroxidized phospholipids of cellular membranes. Liver preneoplastic foci were obtained in rats by the i.p. administration of diethylnitrosamine and of 2-acetylaminofluorene in the diet. Frozen sections of the liver, incubated in the presence of reduced nicotinamide adenine dinucleotide phosphate:iron revealed the presence of areas in which lipid peroxidation had not been induced (Schiff-negative areas). These areas corresponded strictly, in serial sections, to areas that were strongly positive to gamma-glutamyltranspeptidase.
由于已知肿瘤细胞膜已丧失发生脂质过氧化的能力，因此研究脂质过氧化敏感性的丧失是否代表一种与其他已知肿瘤标志物一起出现在癌前细胞中的肿瘤标志物，似乎很有意义。开发了一种组织化学技术来检测暴露于有效促氧化剂的肝脏切片单个细胞中的脂质过氧化。该技术基于使用席夫试剂检测与蛋白质结合的醛（烯醛）。后一种试剂还可以检测细胞膜过氧化磷脂酰基中存在的羰基功能。通过腹腔注射二乙基亚硝胺和在饮食中添加2-乙酰氨基芴在大鼠中获得肝脏癌前病灶。在存在还原型烟酰胺腺嘌呤二核苷酸磷酸：铁的情况下孵育的肝脏冷冻切片显示存在未诱导脂质过氧化的区域（席夫阴性区域）。在连续切片中，这些区域与对γ-谷氨酰转肽酶呈强阳性的区域严格对应。
