Kasten F H, Lala R
Stain Technol. 1975 May;50(3):197-201. doi: 10.3109/10520297509117057.
A technique is described for performing the Feulgen reaction for DNA on cells and tissues fixed in glutaraldehyde. Blockade free aldehydes by reducing them with fresh 0.5% NaBH4 in 1% NaH2PO4 for 1 hr at room temperature, then rinse in water. Follow by a Feulgen reaction (hydrolysis at room temperature in 6 N HCl for 20 min, Schiff's reagent for 60 min.). Controls assure the completeness and irreversibility of the borohydride blockade. Cytophotometry shows that the DNA content per nucleus is unaffected by the blockade procedure.
本文描述了一种对戊二醛固定的细胞和组织进行DNA福尔根反应的技术。用新鲜的1%磷酸二氢钠中的0.5%硼氢化钠在室温下将游离醛还原1小时以封闭醛基，然后用水冲洗。接着进行福尔根反应（在室温下用6N盐酸水解20分钟，用席夫试剂处理60分钟）。对照实验确保了硼氢化物封闭的完整性和不可逆性。细胞光度测定表明每个细胞核的DNA含量不受封闭程序的影响。
