Laboratory Worker Self-Contamination with Noninfectious SARS-CoV-2 DNA Can Result in False-Positive Reverse Transcriptase PCR-Based Surveillance Testing.
作者信息
Montgomery Theresa L, Paavola Michelle, Bruce Emily A, Botten Jason W, Crothers Jessica W, Krementsov Dimitry N
机构信息
Department of Biomedical and Health Sciences, College of Nursing and Health Sciences, University of Vermont, Burlington, Vermont, USA.
Center for Health and Wellbeing, University of Vermont, Burlington, Vermont, USA.
出版信息
J Clin Microbiol. 2021 Jun 18;59(7):e0072321. doi: 10.1128/JCM.00723-21.
Abstract
摘要
相似文献
1
Laboratory Worker Self-Contamination with Noninfectious SARS-CoV-2 DNA Can Result in False-Positive Reverse Transcriptase PCR-Based Surveillance Testing.实验室工作人员被非感染性严重急性呼吸综合征冠状病毒2（SARS-CoV-2）DNA自我污染可导致基于逆转录聚合酶链反应（RT-PCR）的监测检测出现假阳性结果。
J Clin Microbiol. 2021 Jun 18;59(7):e0072321. doi: 10.1128/JCM.00723-21.
2
Comparison of Severe Acute Respiratory Syndrome Coronavirus 2 Screening Using Reverse Transcriptase-Quantitative Polymerase Chain Reaction or CRISPR-Based Assays in Asymptomatic College Students.无症状大学生中使用逆转录-定量聚合酶链反应或基于 CRISPR 的检测方法筛查严重急性呼吸综合征冠状病毒 2 的比较。
JAMA Netw Open. 2021 Feb 1;4(2):e2037129. doi: 10.1001/jamanetworkopen.2020.37129.
3
False negative RT-PCR or false positive serological testing in SARS-CoV-2 diagnostics? Navigating between Scylla and Charybdis to prevent misclassification bias in COVID-19 clinical investigations.新型冠状病毒2诊断中逆转录聚合酶链反应假阴性或血清学检测假阳性？在锡拉岩礁和卡律布狄斯漩涡之间穿行，以防止2019冠状病毒病临床研究中的错误分类偏倚。
Diagnosis (Berl). 2020 Nov 18;7(4):405-407. doi: 10.1515/dx-2020-0091.
4
[SARS-CoV-2 and Microbiological Diagnostic Dynamics in COVID-19 Pandemic].[严重急性呼吸综合征冠状病毒2与2019冠状病毒病大流行中的微生物诊断动态]
Mikrobiyol Bul. 2020 Jul;54(3):497-509. doi: 10.5578/mb.69839.
5
Routine Laboratory Blood Tests Predict SARS-CoV-2 Infection Using Machine Learning.常规实验室血液检测可通过机器学习预测 SARS-CoV-2 感染。
Clin Chem. 2020 Nov 1;66(11):1396-1404. doi: 10.1093/clinchem/hvaa200.
6
Rapid, Sensitive, and Specific Severe Acute Respiratory Syndrome Coronavirus 2 Detection: A Multicenter Comparison Between Standard Quantitative Reverse-Transcriptase Polymerase Chain Reaction and CRISPR-Based DETECTR.快速、敏感、特异的严重急性呼吸综合征冠状病毒 2 检测：基于标准定量逆转录聚合酶链反应和基于 CRISPR 的 DETECTR 的多中心比较。
J Infect Dis. 2021 Feb 3;223(2):206-213. doi: 10.1093/infdis/jiaa641.
7
A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase-Polymerase Chain Reaction and Virus Variation in Guangzhou, China.中国广州一例常规逆转录-聚合酶链反应阴性和病毒变异的 SARS-CoV-2 肺炎确诊病例。
Clin Infect Dis. 2021 Jul 15;73(2):e426-e433. doi: 10.1093/cid/ciaa941.
8
Delayed Laboratory Response to COVID-19 Caused by Molecular Diagnostic Contamination.因分子诊断污染导致对 COVID-19 的实验室反应延迟。
Emerg Infect Dis. 2020 Aug;26(8):1944-1946. doi: 10.3201/eid2608.201843. Epub 2020 May 20.
9
Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratories.临床实验室多重实时 RT-PCR 法用于分子诊断 COVID-19 的高灵敏度 SARS-CoV-2 RNA 检测
Clin Chim Acta. 2020 Aug;507:139-142. doi: 10.1016/j.cca.2020.04.023. Epub 2020 Apr 23.
10
Distribution of SARS-CoV-2 PCR Cycle Threshold Values Provide Practical Insight Into Overall and Target-Specific Sensitivity Among Symptomatic Patients.SARS-CoV-2 PCR 循环阈值分布为有症状患者的整体和目标特异性灵敏度提供了实用见解。
Am J Clin Pathol. 2020 Sep 8;154(4):479-485. doi: 10.1093/ajcp/aqaa133.
引用本文的文献
1
Development of a novel strategy to reduce diagnostic errors in real-time polymerase chain reaction using probe-based techniques.开发一种新策略，使用基于探针的技术减少实时聚合酶链反应中的诊断错误。
Sci Rep. 2024 Nov 8;14(1):27163. doi: 10.1038/s41598-024-78654-2.
2
Surveillance of Vermont wildlife in 2021-2022 reveals no detected SARS-CoV-2 viral RNA.2021-2022 年对佛蒙特州野生动物的监测并未发现可检测到的 SARS-CoV-2 病毒 RNA。
Sci Rep. 2023 Sep 6;13(1):14683. doi: 10.1038/s41598-023-39232-0.
3
Surveillance of Vermont wildlife in 2021-2022 reveals no detected SARS-CoV-2 viral RNA.2021 - 2022年对佛蒙特州野生动物的监测显示未检测到严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒RNA。
bioRxiv. 2023 Apr 26:2023.04.25.538264. doi: 10.1101/2023.04.25.538264.
4
The Role of Surveillance Inspections in Reducing False-Positives of SARS-CoV-2 Omicron Variants during the COVID-19 Epidemic.监测检查在降低新冠疫情期间新冠病毒奥密克戎变异株假阳性方面的作用
Can J Infect Dis Med Microbiol. 2023 Apr 19;2023:8508975. doi: 10.1155/2023/8508975. eCollection 2023.
5
Comparison of the performance of two real-time fluorescent quantitative PCR kits for the detection of SARS-CoV-2 nucleic acid: a study based on large real clinical samples.两种实时荧光定量 PCR 试剂盒检测 SARS-CoV-2 核酸性能的比较：基于大样本临床研究。
Virol J. 2022 Nov 18;19(1):191. doi: 10.1186/s12985-022-01922-y.
6
Predicting infectivity: comparing four PCR-based assays to detect culturable SARS-CoV-2 in clinical samples.预测传染性：比较四种基于 PCR 的检测方法在临床样本中检测可培养 SARS-CoV-2 的效果。
EMBO Mol Med. 2022 Feb 7;14(2):e15290. doi: 10.15252/emmm.202115290. Epub 2021 Dec 13.
7
Laboratory-Generated DNA Can Cause Anomalous Pathogen Diagnostic Test Results.实验室生成的 DNA 可能导致异常的病原体诊断测试结果。
Microbiol Spectr. 2021 Oct 31;9(2):e0031321. doi: 10.1128/Spectrum.00313-21. Epub 2021 Sep 15.
本文引用的文献
1
Anomalous COVID-19 tests hinder researchers.异常的新冠病毒检测结果给研究人员带来了阻碍。
Science. 2021 Jan 15;371(6526):244-245. doi: 10.1126/science.abf8873.
2
SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract.SARS-CoV-2 反向遗传学揭示了呼吸道感染的可变梯度。
Cell. 2020 Jul 23;182(2):429-446.e14. doi: 10.1016/j.cell.2020.05.042. Epub 2020 May 27.
3
An Infectious cDNA Clone of SARS-CoV-2.严重急性呼吸综合征冠状病毒 2 的感染性 cDNA 克隆。
Cell Host Microbe. 2020 May 13;27(5):841-848.e3. doi: 10.1016/j.chom.2020.04.004. Epub 2020 Apr 13.
Zotero 插件