Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada
mSphere. 2021 May 5;6(3):e00365-21. doi: 10.1128/mSphere.00365-21.
Aaron Reinke studies microsporidian evolution and how microsporidia interact with their hosts. In this mSphere of Influence article, he reflects on how the papers "A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells" (K. J. Roux, D. I. Kim, M. Raida, and B. Burke, J Cell Biol 196:801-810, 2012, https://doi.org/10.1083/jcb.201112098) and "Proteomic mapping of mitochondria in living cells via spatially restricted enzymatic tagging" (H.-W. Rhee, P. Zou, N. D. Udeshi, J. D. Martell, et al., Science 339:1328-1331, 2013, https://doi.org/10.1126/science.1230593) impacted his thinking on how to determine where proteins from intracellular pathogens are located within host cells.
亚伦·莱因克研究微孢子虫的进化以及微孢子虫与其宿主的相互作用。在这篇《mSphere 影响力》文章中，他反思了两篇论文“一种混杂的生物素连接酶融合蛋白鉴定哺乳动物细胞中的近端和相互作用蛋白”（K. J. 鲁克斯、D. I. 金、M. 赖达和 B. 伯克，《细胞生物学杂志》196：801-810，2012，https://doi.org/10.1083/jcb.201112098）和“通过空间受限的酶标记对活细胞中线粒体进行蛋白质组映射”（H.-W. 雷、P. 邹、N. D. 乌德希、J. D. 马泰尔等人，《科学》339：1328-1331，2013，https://doi.org/10.1126/science.1230593）如何影响他思考如何确定细胞内病原体的蛋白质在宿主细胞中的位置。
