Women's Environmental Science Laboratory, Department of Health Sciences, Faculty of Human Life and Environment, Nara Women's University.
J Nutr Sci Vitaminol (Tokyo). 2021;67(2):130-134. doi: 10.3177/jnsv.67.130.
Estradiol (E) modulates the central and peripheral thermoregulatory responses to cold. Menthol is an agonist of transient receptor potential melastatin type 8 (TRPM8), which is a peripheral cold receptor. E suppresses menthol-induced elevation of body temperature (T) in ovariectomized rats, but the mechanism is unknown. The aim of the present study is to investigate the effect of E on uncoupling protein 1 (UCP1), a thermogenic gene, and TRPM8 mRNA levels in ovariectomized rats applied menthol. A silastic tube was implanted in ovariectomized rats with and without E underneath the dorsal skin (E(+) and E(-) groups), and data loggers for T measurement into peritoneal cavity. After application of 10% L-menthol or vehicle to the skin of the whole trunk of rats, T was measured for 2 h. The interscapular brown adipose tissue (BAT) and spinal ganglia of cervical, thoracic, and lumbar parts were obtained for RT-qPCR assay. In the menthol application, T in the E(+) group was lower than that in the E(-) group. The UCP1 mRNA in the BAT, TRPM8 mRNA in the BAT and spinal ganglia in all areas did not differ between the E(+) and E(-) groups. In conclusion, the UCP1 and TRPM8 expression in the brown fat did not affect the restriction of the menthol-induced hyperthermia by estradiol in ovariectomized rats.
雌二醇（E）调节对寒冷的中枢和外周体温调节反应。薄荷醇是瞬时受体电位 melastatin 型 8（TRPM8）的激动剂，TRPM8 是外周冷受体。E 抑制去卵巢大鼠中薄荷醇诱导的体温升高（T），但其机制尚不清楚。本研究旨在探讨 E 对解偶联蛋白 1（UCP1），一种产热基因，以及应用薄荷醇的去卵巢大鼠中 TRPM8 mRNA 水平的影响。在去卵巢大鼠的背部皮肤下植入硅橡胶管（E(+)和 E(-)组），并将腹腔内的 T 测量数据记录器植入。在向大鼠整个躯干的皮肤涂抹 10% L-薄荷醇或载体后，测量 T 2 小时。获得肩胛间棕色脂肪组织（BAT）和颈、胸、腰部分的脊神经节进行 RT-qPCR 检测。在薄荷醇应用中，E(+)组的 T 低于 E(-)组。BAT 中的 UCP1 mRNA、BAT 和所有区域脊神经节中的 TRPM8 mRNA 在 E(+)和 E(-)组之间没有差异。总之，棕色脂肪中的 UCP1 和 TRPM8 表达并不影响雌二醇对去卵巢大鼠中薄荷醇诱导的体温升高的限制。
