Kubo Takuro, Tsuji Shunichiro, Amano Tsukuru, Yoshino Fumi, Niwa Yoko, Kasahara Kyoko, Yoshida Saori, Mukaisho Ken-Ichi, Sugihara Hiroyuki, Tanaka Sachiko, Kimura Fuminori, Takahashi Kentaro, Murakami Takashi
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Seta-tsukinowa, Otsu, Shiga 520-2192, Japan.
Department of Pathology, Division of Molecular and Diagnostic Pathology, Shiga University of Medical Science, Seta-tsukinowa, Otsu, Shiga 520-2192, Japan.
Exp Anim. 2017 Oct 30;66(4):337-343. doi: 10.1538/expanim.17-0028. Epub 2017 Jun 19.
Transient receptor potential cation channel subfamily M member 8 (TRPM8) is associated with sensitivity to cold sensation in mammals. A previous study demonstrated that TRPM8 was overexpressed in the skin of ovariectomized (OVX) rats due to the loss of estrogen. In the present study, we investigated whether estrogen replacement restricts overexpression of the TRPM8 channel in the skin of OVX rats. We divided 15 Sprague Dawley rats into three groups: a non-operated group (NON-OPE), an ovariectomy group (OVX), and a group subjected to estrogen replacement during 4 weeks beginning 7 days after ovariectomy (OVX + E2). Five weeks later, TRPM8 channel mRNA and protein in lumbar skin were quantified by real-time RT-PCR, protein ELISA, and immunohistochemistry. The OVX + E2 group exhibited a trend for decreased expression of the TRPM8 channel in the lumbar skin in comparison with the OVX group, whereas ELISA data and immunohistochemistry data and immunohistochemistry graphs relating to TRPM8 protein did not show any obvious differences between the OVX group and the OVX + E2 group. Estrogen replacement may restrict the overexpression of TRPM8 in the dermis of OVX rats.
瞬时受体电位阳离子通道M亚家族成员8（TRPM8）与哺乳动物的冷觉敏感性相关。先前的一项研究表明，由于雌激素缺乏，TRPM8在去卵巢（OVX）大鼠的皮肤中过度表达。在本研究中，我们调查了雌激素替代是否会限制OVX大鼠皮肤中TRPM8通道的过度表达。我们将15只Sprague Dawley大鼠分为三组：未手术组（NON - OPE）、去卵巢组（OVX）和在去卵巢7天后开始进行4周雌激素替代的组（OVX + E2）。五周后，通过实时RT - PCR、蛋白质ELISA和免疫组织化学对腰部皮肤中的TRPM8通道mRNA和蛋白质进行定量。与OVX组相比，OVX + E2组腰部皮肤中TRPM8通道的表达有降低的趋势，而ELISA数据、免疫组织化学数据以及与TRPM8蛋白相关的免疫组织化学图像在OVX组和OVX + E2组之间未显示出任何明显差异。雌激素替代可能会限制OVX大鼠真皮中TRPM8的过度表达。
