Department of Veterinary Clinic, Reproduction and Pathology Faculty of Veterinary Medicine, IPB University, Indonesia.
Research Center for Zoonosis Control (RCZC), Hokkaido University, Japan.
Jpn J Infect Dis. 2022 Jan 24;75(1):83-85. doi: 10.7883/yoken.JJID.2020.769. Epub 2021 Apr 30.
Coxiella burnetii is a bacterial pathogen that causes Q fever, which is widespread worldwide. Livestock such as cattle, goats, and sheep are the main sources of C. burnetii infection. C. burnetii infection causes abortion in livestock, resulting in economic damage. Q fever is a zoonotic disease and a potential public health hazard. To date, little is known about C. burnetii infection in livestock in Indonesia. The objective of this study was to screen the genome of C. burnetii bacteria in beef cattle in West Java, Indonesia. Organ tissue samples were collected from cattle slaughtered in slaughterhouses in West Java. C. burnetii genome was detected in cattle samples obtained from three sampling areas using nested PCR, targeting the com1 gene of C. burnetii. Sequencing analysis of the 16S rRNA gene revealed that the amplicons showed 99.9% nucleotide identity to the C. burnetii strains: Heizberg, 1843, 2574, 701CbB1, and 14160-001. Our results indicate that C. burnetii infection occurs in Indonesian beef cattle and highlight the risk of exposure to C. burnetii infection in humans.
贝氏考克斯体是一种引起 Q 热的细菌病原体，广泛分布于世界各地。牛、山羊和绵羊等牲畜是 C. burnetii 感染的主要来源。C. burnetii 感染会导致牲畜流产，造成经济损失。Q 热是一种人畜共患病，也是潜在的公共卫生危害。迄今为止，人们对印度尼西亚牲畜中 C. burnetii 感染的了解甚少。本研究的目的是筛查印度尼西亚西爪哇省肉牛中的 C. burnetii 细菌基因组。从西爪哇省屠宰场屠宰的牛中采集组织器官样本。使用巢式 PCR 检测来自三个采样区的牛样本中的 C. burnetii 基因组，针对 C. burnetii 的 com1 基因。16S rRNA 基因测序分析显示，扩增子与 C. burnetii 菌株 Heizberg、1843、2574、701CbB1 和 14160-001 的核苷酸同一性为 99.9%。我们的结果表明 C. burnetii 感染存在于印度尼西亚肉牛中，并强调了人类接触 C. burnetii 感染的风险。
