Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Food Hygiene and Quality Control, Division of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Exp Appl Acarol. 2024 Apr;92(3):529-546. doi: 10.1007/s10493-023-00888-y. Epub 2024 Feb 26.
This survey sought to molecularly detect Coxiella burnetii in Argasidae and Ixodidae ticks attached to small ruminants in the region of West Azerbaijan (Northwest of Iran) and blood samples collected from the same animals. 451 tick samples and 927 blood samples were obtained from sheep (n = 536) and goats (n = 391) and tested by nested PCR for detection of C. burnetii insertion sequence IS1111 or icd gene sequence. The collected ticks were morphologically classified as Rhipicephalus sanguineus, Rhipicephalus turanicus, Hyalomma asiaticum, Hyalomma anatolicum, or Argas reflexus. 14% of ticks (65 in total 43 for IS1111 and 22 for icd gene) tested positive for C. burnetii, none of which were from the Argas genus. Among the 927 blood samples, 218 (23.5%) tested positive for C. burnetii. The positive result from analysis targeting the genes IS1111 and icd were 131 and 87 respectively. As Q fever is a tickborne zoonosis and endemic to Iran, such information is critical for creating effective, coordinated, and strategic tick and pathogen control programs to prevent disease outbreak in domestic animals and humans.
本研究旨在通过巢式 PCR 检测西北伊朗东阿塞拜疆地区附着于小反刍动物的璃眼蜱属和硬蜱属蜱以及从同一动物采集的血液样本中是否存在贝氏柯克斯体。从绵羊（n=536）和山羊（n=391）中采集了 451 个蜱样本和 927 个血液样本，用于检测 C. burnetii 插入序列 IS1111 或 icd 基因序列。采集的蜱虫经形态学分类为 Rhipicephalus sanguineus、Rhipicephalus turanicus、Hyalomma asiaticum、Hyalomma anatolicum 或 Argas reflexus。14%的蜱虫（总共 65 只，43 只为 IS1111，22 只为 icd 基因）检测出 C. burnetii 阳性，均不属于 Argas 属。在 927 个血液样本中，有 218 个（23.5%）检测出 C. burnetii 阳性。针对 IS1111 和 icd 基因的阳性结果分别为 131 和 87。由于 Q 热是一种蜱传动物传染病，且在伊朗流行，因此这些信息对于制定有效的、协调的和战略性的蜱和病原体控制计划以预防家畜和人类的疾病爆发至关重要。
