Department of Gynecology, Affiliated Hospital of Hebei University.
J Oleo Sci. 2021;70(5):675-683. doi: 10.5650/jos.ess21023.
Cyclophosphamide (CP) is very well-known anticancer drug and commonly used against various cancers. CP therapy is related to female ovarian cancer and causes female infertility. The ovarian cancer associated with the increase oxidative stress and inflammatory reaction. Syringic acid (SA) is very well phyto-constituent and already proof antioxidant and anti-inflammatory effects on various diseases. We investigated the chemoprotective impact of SA on CP mediated ovarian damage, and the underlying mechanism. CP (75 mg/kg) was used to cause ovarian damage and rats were randomly divided into separate groups and received a different dose of SA for 14-day. Body weight, food and water intake were determined. Ovarian weight and tumor index was measured. Antioxidant parameters were determined in the serum and ovarian tissue. Pro-inflammatory cytokines, apoptosis parameters and inflammatory mediators were estimated in the serum. Hormonal parameters and Histomorphometry were estimated. Dose dependently treatment of SA significantly (p < 0.001) decreased the levels of biochemical parameter such as nitric oxide (NO), myeloperoxidase (MPO) and augmented the antioxidant parameters include catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and reduced malondialdehyde (MDA) level in serum and ovarian tissue. SA treatment significantly (p < 0.001) suppressed the level of luteinizing hormones (LH), anti-mullerian hormone (AMH), estradiol (E2) and folliclestimulating hormone (FSH) as well as ovarian follicles. SA significantly (p < 0.001) down-regulated cytokines, inflammatory mediator and caspase-3 parameters. Taken altogether, we conclude that SA considerably reduced ovarian damage via reduced oxidative stress and inflammatory reaction.
环磷酰胺（CP）是一种非常著名的抗癌药物，常用于治疗各种癌症。CP 治疗与女性卵巢癌有关，并导致女性不孕。卵巢癌与氧化应激和炎症反应的增加有关。丁香酸（SA）是一种很好的植物成分，已经证明对各种疾病具有抗氧化和抗炎作用。我们研究了 SA 对 CP 介导的卵巢损伤的化学保护作用及其潜在机制。用 CP（75mg/kg）引起卵巢损伤，将大鼠随机分为单独的组，并在 14 天内接受不同剂量的 SA 治疗。测定体重、食物和水的摄入量。测量卵巢重量和肿瘤指数。测定血清和卵巢组织中的抗氧化参数。估计血清中的促炎细胞因子、凋亡参数和炎症介质。估计激素参数和组织形态计量学。SA 的剂量依赖性治疗显著（p<0.001）降低了生化参数如一氧化氮（NO）、髓过氧化物酶（MPO）的水平，并增强了抗氧化参数，包括过氧化氢酶（CAT）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和降低丙二醛（MDA）水平在血清和卵巢组织中。SA 治疗显著（p<0.001）抑制了黄体生成素（LH）、抗苗勒氏管激素（AMH）、雌二醇（E2）和卵泡刺激素（FSH）以及卵巢卵泡的水平。SA 显著（p<0.001）下调细胞因子、炎症介质和半胱天冬酶-3 参数。总的来说，我们得出结论，SA 通过减少氧化应激和炎症反应，显著减轻卵巢损伤。
