Miasishcheva N V, Golenko O D, Kuznetsova L E, Raushenbakh M O, Rudakova I P
Vopr Med Khim. 1977 Sep-Oct;23(5):622-9.
Comparative analysis of the functional activity of several fluoralkylcobalamines was carried out using E. coli 113/3 strain deficient in vitamin B12 and methionine. Difluoro chlor methylcobalamine (CF2Cl-Cbl) exhibited the most distinct inhibitory effect on growth of bacterial cells in the medium with cobalamine. Effect of methylcobalamine and CF2Cl-Cbl on the proliferative activity of human embryonal fibroblasts was studied in media of various composition. The proliferative activity of fibroblasts was distinctly increased in the medium with methylcobalamine at various periods of cultivation; the fraction of 3H-thimidine labelled cells and the mitotic index were increased. The distinct decrease in amount of cells, synthesizing DNA, and in their mitotic activity was observed in medium with CF2Cl-Cbl. The data obtained suggest that difluorochlor methylcobalamine affects the cell proliferation as the antagonist of methylcobalamine in experiments with bacterial cells and the primary culture of human embryonal fibroblasts.
利用缺乏维生素B12和蛋氨酸的大肠杆菌113/3菌株，对几种氟烷基钴胺素的功能活性进行了比较分析。二氟氯甲基钴胺素（CF2Cl-Cbl）在含有钴胺素的培养基中对细菌细胞生长表现出最明显的抑制作用。在不同组成的培养基中研究了甲基钴胺素和CF2Cl-Cbl对人胚胎成纤维细胞增殖活性的影响。在不同培养时期，含有甲基钴胺素的培养基中，成纤维细胞的增殖活性明显增加；3H-胸腺嘧啶标记细胞的比例和有丝分裂指数增加。在含有CF2Cl-Cbl的培养基中，观察到合成DNA的细胞数量及其有丝分裂活性明显下降。所得数据表明，在细菌细胞和人胚胎成纤维细胞原代培养实验中，二氟氯甲基钴胺素作为甲基钴胺素的拮抗剂影响细胞增殖。
