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[钕激光照射后成纤维细胞培养的放射自显影研究]

[Radioautographic study of a culture of fibroblasts after irradiation by a neodymium laser].

作者信息

Moskalik K G, Kniazev P G, Lazo V V

出版信息

Arkh Anat Gistol Embriol. 1979 Mar;76(3):28-34.

PMID:435098
Abstract

By means of radioautographic method, with 3H-thymidin application there were studied: mitotic activity, the number of DNA-synthesizing cells, growth fraction and population number of fibroblast culture irradiated with a single impulse of neodymium laser at energy density within 1 Dg/cm2--100 Dg/cm2. Under the influence of small doses of laser radiation (1--10 Dg/cm2) the number of dividing DNA-synthesizing cells increased, together with proliferative pool and the number of cells in the field of vision. At energy density of laser radiation equal to 50 Dg/cm2, and especially to 100 Dg/cm2 despite increased mitotic index and labelled cells index, registered during first hours of the effect, proliferative pool decreased and population growth rate lowered. These doses of laser radiation produced destruction of a rather large part of the cells in a monolayer.

摘要

采用放射自显影法,通过应用³H-胸腺嘧啶核苷,研究了在能量密度为1 Dg/cm²至100 Dg/cm²范围内用钕激光单次脉冲照射的成纤维细胞培养物的有丝分裂活性、DNA合成细胞数量、生长分数和群体数量。在小剂量激光辐射(1至10 Dg/cm²)的影响下,进行DNA合成的分裂细胞数量增加,增殖池和视野中的细胞数量也增加。当激光辐射的能量密度等于50 Dg/cm²,特别是等于100 Dg/cm²时,尽管在效应的最初几个小时观察到有丝分裂指数和标记细胞指数增加,但增殖池减少,群体生长速率降低。这些剂量的激光辐射导致单层培养物中相当一部分细胞被破坏。

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