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[小鼠脾脏肿瘤细胞中钴胺素辅酶的分析]

[Analysis of the cobalamin coenzymes in mouse splenic tumor cells].

作者信息

Vares Iu V, Miasishcheva N V

出版信息

Vopr Med Khim. 1977 Sep-Oct;23(5):681-4.

PMID:339530
Abstract

Coabalamine coenzymes were studied in tumor spleen cells of mice with La leukosis. Endogenous cobalamines in the cell extracts were separated by two-dimensional thin-layer chromatography and by bioautography. Analysis of the cobalamines ratio was carried out using bioautochromatographic technique. 5-deoxyladenosyl- and methyl cobalamines were found in extracts of the tumor cells.

摘要

对患有La白血病的小鼠肿瘤脾细胞中的钴胺素辅酶进行了研究。通过二维薄层色谱法和生物自显影法分离细胞提取物中的内源性钴胺素。使用生物自显影色谱技术对钴胺素比例进行分析。在肿瘤细胞提取物中发现了5-脱氧腺苷钴胺素和甲基钴胺素。

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