Seifert R, Schächtele C, Rosenthal W, Schultz G
Institut für Pharmakologie, Freie Universität Berlin, F.R.G.
Biochem Biophys Res Commun. 1988 Jul 15;154(1):20-6. doi: 10.1016/0006-291x(88)90643-2.
Both cis- and trans-unsaturated but not saturated fatty acids activated protein kinase C purified to apparent homogeneity from rat brain. Fatty-acid-induced enzyme activation was not more than additive with that by phospholipids and was potentiated by diacylglycerol. Recently, we demonstrated that cis- and trans-unsaturated fatty acids induced platelet aggregation and phosphorylation of specific proteins. Both events were potentiated by a cell-permeable diacylglycerol [(1987) Biochem. Biophys. Res. Commun. 149, 762-768]. Thus, trans-unsaturated fatty acids may provide useful experimental tools for the study of protein kinase C activation in vitro and in vivo. Our results suggest that fatty acids and diacylglycerol may synergistically be involved in hormonal stimulation of protein kinase C, as certain hormonal stimuli cause release of diacylglycerol and fatty acids from phospholipids by parallel activation of phospholipases C and A2.
顺式和反式不饱和脂肪酸而非饱和脂肪酸，能激活从大鼠脑中纯化至表观均一性的蛋白激酶C。脂肪酸诱导的酶激活作用与磷脂诱导的激活作用相加不超过此水平，且二酰甘油可增强该激活作用。最近，我们证明顺式和反式不饱和脂肪酸可诱导血小板聚集及特定蛋白质的磷酸化。这两个事件均被一种细胞可渗透的二酰甘油增强[（1987年）《生物化学与生物物理学研究通讯》149, 762 - 768]。因此，反式不饱和脂肪酸可能为体外和体内蛋白激酶C激活的研究提供有用的实验工具。我们的结果表明，脂肪酸和二酰甘油可能协同参与蛋白激酶C的激素刺激，因为某些激素刺激通过平行激活磷脂酶C和A2，导致从磷脂中释放二酰甘油和脂肪酸。
