Go M, Sekiguchi K, Nomura H, Kikkawa U, Nishizuka Y
Biochem Biophys Res Commun. 1987 Apr 29;144(2):598-605. doi: 10.1016/s0006-291x(87)80008-6.
Specificity of 1,2-diacylglycerol for the activation of protein kinase C was investigated with various synthetic products. 1-Stearoyl-2-arachidonylglycerol, a major species of diacylglycerol derived from the receptor-mediated hydrolysis of inositol phospholipids, was most active, but many other diacylglycerols having naturally occurring fatty acids were almost equally active in this role. Hormone-sensitive lipase could produce potentially active diacylglycerols during lipolysis. The lack of the specificity may be reconciled with the possibility that the stearoyl-arachidonyl species is the diacylglycerol with which protein kinase C indeed comes in contact in the membrane when the receptor is stimulated, and that diacylglycerols from other sources are produced in distinct compartments and are not intercalated into the phospholipid bilayer.
利用各种合成产物研究了1,2 - 二酰基甘油激活蛋白激酶C的特异性。1 - 硬脂酰 - 2 - 花生四烯酰甘油是由受体介导的肌醇磷脂水解产生的主要二酰基甘油种类，其活性最高，但许多含有天然脂肪酸的其他二酰基甘油在这一作用中几乎具有同等活性。激素敏感脂肪酶在脂肪分解过程中可产生潜在活性的二酰基甘油。这种特异性的缺乏可能与以下可能性相协调：当受体受到刺激时，硬脂酰 - 花生四烯酰种类是蛋白激酶C在膜中实际接触的二酰基甘油，而来自其他来源的二酰基甘油在不同的区室中产生，并且不会插入磷脂双层中。
