Saito Y, Hirashima N, Kirino Y
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Biochem Biophys Res Commun. 1988 Jul 15;154(1):85-90. doi: 10.1016/0006-291x(88)90653-5.
When sarcoplasmic reticulum membrane vesicles or synaptosomes were mixed with sonicated phospholipid vesicles and subjected to freezing-thawing, giant vesicles of up to 50 microns in diameter were formed. When the biomembrane vesicles were labeled with a covalently binding fluorescent dye, the resultant giant vesicles were fluorescent, thereby suggesting that the freezing-thawing process induces fusion of phospholipid and biomembrane vesicles. When membranes of the giant proteoliposomes thus prepared were studied using the patch-clamp technique, potassium channels of the biomembranes were detectable. The present method of the giant proteoliposome preparation is simple and rapid, and provides a system suitable for the study of ion channels of various biomembranes usually inaccessible to a patch-pipette microelectrode.
当肌浆网膜囊泡或突触体与超声处理过的磷脂囊泡混合并进行冻融处理时，会形成直径达50微米的巨大囊泡。当生物膜囊泡用共价结合的荧光染料标记时，所得的巨大囊泡具有荧光，从而表明冻融过程会诱导磷脂囊泡与生物膜囊泡融合。当使用膜片钳技术研究如此制备的巨大蛋白脂质体的膜时，可检测到生物膜的钾通道。目前制备巨大蛋白脂质体的方法简单快速，且提供了一个适合研究各种通常无法用膜片吸管微电极接触的生物膜离子通道的系统。
