Criado M, Keller B U
Max-Planck-Institut für biophysikalische Chemie, Abteilung Membranbiophysik, Göttingen, FRG.
FEBS Lett. 1987 Nov 16;224(1):172-6. doi: 10.1016/0014-5793(87)80442-8.
Membranes of cellular organelles and plasma membranes of some type of cells are not accessible to the high-resolution recordings that the conventional patch-clamp technique allows. However, when these purified membranes are dehydrated together with small lipid vesicles and hydrated again, cell-size vesicles (5-100 micron diameter) are obtained, on which single-channel recordings are possible. This approach, which has been proven successful with about ten different membrane preparations of varied origin, is further illustrated with two examples. First, a known conductivity of the sarcoplasmic reticulum membrane is compared with data obtained by using other techniques. Second, a new sodium current, present at purified postsynaptic membranes from the Torpedo electric organ, is described.
某些类型细胞的细胞器膜和质膜无法进行传统膜片钳技术所允许的高分辨率记录。然而，当这些纯化的膜与小脂质囊泡一起脱水然后再次水合时，可获得细胞大小的囊泡（直径5 - 100微米），在其上可以进行单通道记录。这种方法已在大约十种不同来源的膜制剂中被证明是成功的，下面用两个例子进一步说明。首先，将肌浆网膜的已知电导率与使用其他技术获得的数据进行比较。其次，描述了在来自电鳐电器官的纯化突触后膜上存在的一种新的钠电流。
