Khamse Safoura, Haftcheshmeh Saeed Mohammadian, Sadr Seyed Shahabeddin, Roghani Mehrdad, Kamalinejad Mohammad, Moghaddam Parvane Mohseni, Golchoobian Ravieh, Ebrahimi Fatemeh
Electrophysiology Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, I.R. Iran.
Department of Medical Immunology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I.R. Iran.
Res Pharm Sci. 2020 Dec 30;16(1):48-57. doi: 10.4103/1735-5362.305188. eCollection 2021 Feb.
Epilepsy is recognized as a chronic neurologic disease. Increasing evidence has addressed the antioxidant and anti-inflammatory roles of olive leaf extract (OLE) in neurodegenerative diseases. So, the current study aimed to investigate the neuroprotective roles of OLE in epilepsy.
Forty rats were divided into 4 groups including a control group, sham group, kainic acid (KA) group, and KA + OLE group. KA (4 μg/rat) was injected intrahippocampal, and OLE (300 mg/kg) was orally administrated for 4 weeks. Animals were sacrificed, and their hippocampi were isolated. KA- induced seizure activity was recorded. Oxidative stress index was assessed by measuring its indicators including malondialdehyde (MDA), nitrite, nitrate, and glutathione (GSH) as well as the catalase (CAT) activity. The supernatant concentration of tumor necrosis factor-α (TNF-α) and the apoptosis rate in neurons were measured.
FINDINGS/RESULTS: Treatment with OLE significantly reduced the seizure score. OLE decreased oxidative stress index by reducing the concentration of MDA, nitrite, and nitrate as well as increasing the level of GSH. OLE had a significant anti-apoptotic effect on neurons. However, CAT activity and the level of TNF-α were not affected.
Our findings indicated neuroprotective properties of OLE, which is mainly mediated by its antioxidant and anti-apoptotic effects, therefore, could be considered as a valuable therapeutic supplement for epilepsy.
癫痫被认为是一种慢性神经疾病。越来越多的证据表明橄榄叶提取物（OLE）在神经退行性疾病中具有抗氧化和抗炎作用。因此，本研究旨在探讨OLE在癫痫中的神经保护作用。
将40只大鼠分为4组，包括对照组、假手术组、 kainic酸（KA）组和KA + OLE组。将KA（4μg/只大鼠）海马内注射，OLE（300mg/kg）口服给药4周。处死动物，分离海马。记录KA诱导的癫痫发作活动。通过测量其指标包括丙二醛（MDA）、亚硝酸盐、硝酸盐和谷胱甘肽（GSH）以及过氧化氢酶（CAT）活性来评估氧化应激指数。测量肿瘤坏死因子-α（TNF-α）的上清液浓度和神经元的凋亡率。
OLE治疗显著降低癫痫发作评分。OLE通过降低MDA、亚硝酸盐和硝酸盐的浓度以及提高GSH水平来降低氧化应激指数。OLE对神经元具有显著的抗凋亡作用。然而，CAT活性和TNF-α水平未受影响。
我们的研究结果表明OLE具有神经保护特性，其主要通过抗氧化和抗凋亡作用介导，因此，可被视为癫痫的一种有价值的治疗补充剂。
