Yuan Wen, Wang Hongbo, Gong Yanling
Department of Pharmacy, College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, China.
Key Laboratory of Pharmaceutical Research for Metabolic Diseases, Qingdao University of Science and Technology, Qingdao, China.
Iran J Basic Med Sci. 2021 Feb;24(2):248-255. doi: 10.22038/ijbms.2020.47599.10973.
Nausea and vomiting are perennial problems in cancer patients undergoing chemotherapy. Orexin-A (OXA) has been shown to regulate feeding and gastric motility. Seabuckthorn ( L.) seed oil (SSO) has been proved to promote digestion and bowel movements. We investigated whether SSO alleviated cisplatin-induced vomiting and its possible mechanism involved in OXA.
Rats were randomly divided into normal control group (NCG), cisplatin group (CG), SSO low-dose group (SLG), SSO middle-dose group (SMG) SSO high-dose group (SHG), and ondansetron group (OG). Rats were pretreated respectively with SSO (0.850, 1.675, and 3.350 g/kg·BW) and ondansetron (2 mg/kg·BW) in SLG, SMG, SHG, and OG for 6 days, and the same volume of saline in NCG and CG groups. On the 6th day, cisplatin (6 mg/kg, IP) was administered in all groups except NCG. The cumulative food and kaolin intake, gastric emptying, plasma OXA level, OXR mRNA and protein expression in the hypothalamus and brainstem, and OXA expression in the lateral hypothalamic area (LHA) were observed, and the HPLC method was used to analyze the composition of SSO.
Kaolin intake in cisplatin-induced vomiting rats was significantly reduced (<0.05) and gastric emptying delayed by cisplatin was improved (<0.05-0.01) by pretreatment with SSO. Plasma OXA concentration, OXR expression in the hypothalamus and brainstem increased significantly (<0.05-0.01). Furthermore, OXA expression in LHA also increased significantly (<0.05).
SSO prevents cisplatin-induced vomiting in rats, which is possibly involved in increasing peripheral and central OXA and the expression of OXR in the hypothalamus and brainstem.
恶心和呕吐是接受化疗的癌症患者长期面临的问题。食欲素-A（OXA）已被证明可调节进食和胃动力。沙棘籽油已被证实能促进消化和肠道蠕动。我们研究了沙棘籽油是否能减轻顺铂诱导的呕吐及其与食欲素-A相关的可能机制。
将大鼠随机分为正常对照组（NCG）、顺铂组（CG）、沙棘籽油低剂量组（SLG）、沙棘籽油中剂量组（SMG）、沙棘籽油高剂量组（SHG）和昂丹司琼组（OG）。SLG、SMG、SHG和OG组大鼠分别用沙棘籽油（0.850、1.675和3.350 g/kg·体重）和昂丹司琼（2 mg/kg·体重）预处理6天，NCG组和CG组给予相同体积的生理盐水。第6天，除NCG组外，所有组均腹腔注射顺铂（6 mg/kg）。观察累积食物和高岭土摄入量、胃排空、血浆食欲素-A水平、下丘脑和脑干中食欲素受体mRNA和蛋白表达以及下丘脑外侧区（LHA）中食欲素-A表达，并采用高效液相色谱法分析沙棘籽油的成分。
沙棘籽油预处理可使顺铂诱导呕吐大鼠的高岭土摄入量显著减少（<0.05），并改善顺铂所致的胃排空延迟（<0.05 - 0.01）。血浆食欲素-A浓度、下丘脑和脑干中食欲素受体表达显著增加（<0.05 - 0.01）。此外，LHA中食欲素-A表达也显著增加（<0.05）。
沙棘籽油可预防大鼠顺铂诱导的呕吐，这可能与增加外周和中枢食欲素-A以及下丘脑和脑干中食欲素受体的表达有关。
