Ahmadi Ebrahim, Khajehali Jahangir
Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
J Arthropod Borne Dis. 2020 Dec 31;14(4):344-352. doi: 10.18502/jad.v14i4.5271. eCollection 2020 Dec.
Insecticide resistance is one of the most important problems associated with the control of , due to the potential of the rapid development of resistance to different chemical insecticides. The present study was carried out to evaluate dichlorvos resistance in the house fly populations collected from central regions of Iran, Isfahan Province and Chaharmahal and Bakhtiari Province, during 2017 to 2019.
Bioassays were carried out using a standard topical application method as well as a fumigation method. The Koohrang population (susceptible) with the lowest LD values to dichlorvos was chosen to calculate the resistance ratios (RR). Altered sensitivity of acetylcholinesterase (AChE), a target enzyme for dichlorvos, was investigated.
According to the results, very high levels of dichlorvos resistance were observed in the Mobarake population (RR= 80.25-fold by topical application and 33-fold by fumigation bioassay), and Isfahan population (RR= 107.30-fold by topical application and 43-fold by fumigation bioassay) compared to the Koohrang population. Acetylcholinesterase of the Koohrang population was the most sensitive to inhibition by dichlorvos based on the determination of median inhibitory concentration (IC), but AChE of Mobarake and Isfahan populations were 741.93- and 343.94- fold less sensitive to inhibition.
The insensitivity of AChE was possibly involved in dichlorvos resistance in the house fly populations.
由于对不同化学杀虫剂的抗性可能迅速发展,杀虫剂抗性是与控制[某种昆虫,原文未明确]相关的最重要问题之一。本研究旨在评估2017年至2019年期间从伊朗中部地区、伊斯法罕省以及恰哈马哈勒和巴赫蒂亚里省采集的家蝇种群对敌敌畏的抗性。
采用标准的点滴法和熏蒸法进行生物测定。选择对敌敌畏LD值最低的库赫朗种群(敏感种群)来计算抗性比率(RR)。研究了敌敌畏的靶标酶乙酰胆碱酯酶(AChE)的敏感性变化。
结果显示,与库赫朗种群相比,莫巴拉克种群(点滴法RR = 80.25倍,熏蒸法生物测定RR = 33倍)和伊斯法罕种群(点滴法RR = 107.30倍,熏蒸法生物测定RR = 43倍)对敌敌畏表现出非常高的抗性水平。根据半数抑制浓度(IC)的测定,库赫朗种群的乙酰胆碱酯酶对敌敌畏抑制最为敏感,但莫巴拉克和伊斯法罕种群的AChE对抑制的敏感性分别低741.93倍和343.94倍。
AChE的不敏感性可能与家蝇种群对敌敌畏的抗性有关。